Detailed information of TA system TA143476

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1630573..1631138
• Replicon: chromosome
• Accession: NZ_CP046058
• Organism: Microcystis aeruginosa NIES-298

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2H6BV23
• Locus tag: D3800_RS07840
• Protein ID: WP_024968549.1
• Coordinates: 1630860..1631138 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D3800_RS07835
• Protein ID: WP_002767514.1
• Coordinates: 1630573..1630860 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3800_RS07805	1625655..1626341	+	687	WP_103112766.1	hypothetical protein	-
D3800_RS07810	1626583..1628010	+	1428	WP_004158996.1	UDP-N-acetylmuramate--L-alanine ligase	-
D3800_RS07815	1628110..1628988	+	879	WP_103112767.1	UDP-N-acetylmuramate dehydrogenase	-
D3800_RS07820	1628989..1629534	-	546	WP_016514523.1	hypothetical protein	-
D3800_RS07825	1629564..1630010	-	447	WP_103112768.1	hypothetical protein	-
D3800_RS07830	1630017..1630286	-	270	WP_103112841.1	hypothetical protein	-
D3800_RS07835	1630573..1630860	-	288	WP_002767514.1	HigA family addiction module antidote protein	Antitoxin
D3800_RS07840	1630860..1631138	-	279	WP_024968549.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D3800_RS07845	1631442..1631834	-	393	WP_103112769.1	ACP S-malonyltransferase	-
D3800_RS07850	1631831..1632208	-	378	WP_002778608.1	DUF433 domain-containing protein	-
D3800_RS07855	1632422..1632799	-	378	WP_002736741.1	hypothetical protein	-
D3800_RS07860	1632796..1633068	-	273	WP_002736754.1	hypothetical protein	-
D3800_RS07870	1633508..1634320	-	813	WP_103112770.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-66)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10369.75 Da
• Isoelectric Point: 7.3596

>T143476 WP_024968549.1 NZ_CP046058:c1631138-1630860 [Microcystis aeruginosa NIES-298]
MIVSFKHRGLEQFFESGTTRGIQANHAQRIRLILARLSAATSPQDMNLPGLVLHELAGQRKGTWTVRVSGNWRITFTFDG
VDACDVDLEDYH

Nucleotide

• Length: 279 bp

>T143476 NZ_CP061108:1849980-1850231 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10511.20 Da
• Isoelectric Point: 7.1657

>AT143476 WP_002767514.1 NZ_CP046058:c1630860-1630573 [Microcystis aeruginosa NIES-298]
MKMHNPPHPGEVLKELCLEPLNLTVTEAAEALGVSRKTLSAILNGRAGISPEMAIRLGKAFDTSPESWLNQQMQYDLWQA
EQTIGNIKVKRLSVV

Nucleotide

• Length: 288 bp

>AT143476 NZ_CP061108:1850228-1850482 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A2H6BV23