Detailed information of TA system TA143458

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3625701..3626347
• Replicon: chromosome
• Accession: NZ_CP046048
• Organism: Proteus mirabilis strain HN2p

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8E4FP08
• Locus tag: GJR66_RS16785
• Protein ID: WP_060555121.1
• Coordinates: 3625701..3626048 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A8E4FP64
• Locus tag: GJR66_RS16790
• Protein ID: WP_060555120.1
• Coordinates: 3626048..3626347 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJR66_RS16750	3620805..3621098	-	294	WP_049218683.1	YqjK-like family protein	-
GJR66_RS16755	3621095..3621456	-	362	Protein_3247	phage holin family protein	-
GJR66_RS16760	3621776..3622975	-	1200	WP_049218680.1	lipoprotein-releasing ABC transporter permease subunit LolC	-
GJR66_RS16765	3623277..3623678	+	402	WP_049218678.1	PPC domain-containing protein	-
GJR66_RS16770	3624095..3624496	-	402	WP_060555124.1	phage holin family protein	-
GJR66_RS16775	3624578..3624931	-	354	WP_060555123.1	hypothetical protein	-
GJR66_RS16780	3625003..3625251	-	249	WP_060555122.1	DUF883 family protein	-
GJR66_RS16785	3625701..3626048	+	348	WP_060555121.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GJR66_RS16790	3626048..3626347	+	300	WP_060555120.1	XRE family transcriptional regulator	Antitoxin
GJR66_RS16795	3626468..3626656	+	189	WP_060555126.1	DUF5397 family protein	-
GJR66_RS16800	3626660..3626938	+	279	WP_060555119.1	virulence factor	-
GJR66_RS16805	3627405..3628895	+	1491	WP_060555118.1	YadA-like family protein	-
GJR66_RS16810	3629082..3629156	-	75	Protein_3258	IS200/IS605 family transposase	-
GJR66_RS16815	3629150..3629257	-	108	Protein_3259	transposase	-
GJR66_RS16820	3629307..3629513	-	207	WP_060555117.1	hypothetical protein	-
GJR66_RS16825	3629510..3630133	-	624	WP_060555116.1	ParA family protein	-
GJR66_RS16830	3630602..3631120	-	519	WP_060555115.1	ClbS/DfsB family four-helix bundle protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(J) / blaCMY-2	-	3483515..3670314	186799

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13561.55 Da
• Isoelectric Point: 8.8337

>T143458 WP_060555121.1 NZ_CP046048:3625701-3626048 [Proteus mirabilis]
MWAVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRLYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYEKLVRIAEDEFTAHLNTLESK

Nucleotide

• Length: 348 bp

>T143458 NZ_CP061101:3952404-3952556 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGTGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11142.01 Da
• Isoelectric Point: 5.8962

>AT143458 WP_060555120.1 NZ_CP046048:3626048-3626347 [Proteus mirabilis]
MRTLDEVIASRSPESQTRIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNELKLATLKRYIEAMG
GKLSLDVELPTGKRVAFHL

Nucleotide

• Length: 300 bp

>AT143458 NZ_CP061101:c3952355-3952298 [Escherichia coli]
GTTCAGCATATAGGGGGCCTCGGGTTGATAGTAAAATATCACTCGGGGCTTTTCTCTA