Detailed information of TA system TA143441

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 11386..11932
• Replicon: plasmid p19110F47-1
• Accession: NZ_CP046043
• Organism: Acinetobacter towneri strain 19110F47

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GJD93_RS00050
• Protein ID: WP_154320108.1
• Coordinates: 11386..11667 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GJD93_RS00055
• Protein ID: WP_004676016.1
• Coordinates: 11660..11932 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJD93_RS00035	7003..9861	-	2859	WP_154320106.1	relaxase/mobilization nuclease domain-containing protein	-
GJD93_RS00040	9858..10181	-	324	WP_154320107.1	hypothetical protein	-
GJD93_RS00045	10894..11268	+	375	WP_004676020.1	hypothetical protein	-
GJD93_RS00050	11386..11667	+	282	WP_154320108.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GJD93_RS00055	11660..11932	+	273	WP_004676016.1	helix-turn-helix domain-containing protein	Antitoxin
GJD93_RS00060	12017..13342	-	1326	Protein_11	ISNCY family transposase	-
GJD93_RS00065	13545..14333	-	789	WP_154320109.1	hypothetical protein	-
GJD93_RS00070	14300..14773	-	474	WP_154320110.1	PD-(D/E)XK nuclease family protein	-
GJD93_RS00075	14843..15406	+	564	WP_154320111.1	macro domain-containing protein	-
GJD93_RS00080	15534..15848	-	315	WP_023274838.1	BrnA antitoxin family protein	-
GJD93_RS00085	15835..16122	-	288	WP_086384391.1	BrnT family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaNDM-1 / aph(3')-VI / aac(3)-IId	-	1..47094	47094

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (28-89)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10640.18 Da
• Isoelectric Point: 7.9862

>T143441 WP_154320108.1 NZ_CP046043:11386-11667 [Acinetobacter towneri]
MLLVSDEEYRQLQQDLLVQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVNEDDQIFFLVAYPKSVKDNLTDKET
AILRQLVKEQFHG

Nucleotide

• Length: 282 bp

>T143441 NZ_CP061097:21761-22111 [Roseomonas marmotae]
ATGCCGGGCTTTGAGGTCTGGGACCTGGTGAAGGTCCCCTTTCCCTACACCAACCGCCCCGTGCAGCAGCGCCGCCCGGC
CCTGGTGATCGCGGCGCCAGAAGCCTCCGGCACGCCAGCGCTGCTCTGGGTGCTGATGGTGACCAGTGCCGAGAACCGTG
GCTGGCCCGGGGATGTGATGGTCTCGGACCTGACCGAGGCTGGGCTGCCGGCTCCTTCTGTCGTGCGCCCCGCCAAGATC
GCCACCATCGAAGCTGGCGATGCGGAGCGGATCGGGCGTCTGCCGGAGCCAGACCAGCGGCAGGTCGCCCAGGCACTCCG
CGCCAGCCTCGGCGGGGTATTGGCGCAGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10178.56 Da
• Isoelectric Point: 7.3200

>AT143441 WP_004676016.1 NZ_CP046043:11660-11932 [Acinetobacter towneri]
MDNNLFDDLVASIKEAGAIKRKEIQASRTTELELPDIKEVREKTGLSQSEFAARLHISHRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT143441 NZ_CP061097:21556-21774 [Roseomonas marmotae]
ATGATCACCAGCCGGCTCACCAGCAAAGCCCAGACGACGATCCCCCAGCCGGTCCGGGCGGCCTTGCGTCTGCGGGAGGG
GGATGAGATCGCCTATACGATCGAGCAGGGGCGGGTCGTGCTGACCCGCGTCCAGCCGGAGCGCGCCGCCGACGATCCGT
TCCGCACCTTTGCCGAATGGGAAAGCCCGGCCGACGCCAAGGCCTATGCCGGGCTTTGA