Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3652880..3653421 Replicon chromosome
Accession NZ_CP046025
Organism Escherichia coli strain C439

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag GJH08_RS17855 Protein ID WP_000615983.1
Coordinates 3653143..3653421 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag GJH08_RS17850 Protein ID WP_000729703.1
Coordinates 3652880..3653140 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJH08_RS17830 3648555..3649340 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
GJH08_RS17835 3649312..3651024 + 1713 Protein_3486 flagellar biosynthesis protein FlhA -
GJH08_RS17840 3651248..3651745 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
GJH08_RS17845 3651921..3652670 - 750 WP_000056849.1 C40 family peptidase -
GJH08_RS17850 3652880..3653140 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
GJH08_RS17855 3653143..3653421 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
GJH08_RS17860 3653577..3654317 + 741 WP_001225679.1 murein L,D-transpeptidase -
GJH08_RS17865 3654288..3655055 - 768 WP_000333380.1 class II glutamine amidotransferase -
GJH08_RS17870 3655261..3655839 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T143420 WP_000615983.1 NZ_CP046025:3653143-3653421 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T143420 NZ_CP061082:3310797-3310979 [Serratia liquefaciens]
GTGAAAAGTTCAGAACTGATCGGGCTATTAGAACAGGAAGGTTGGGTTTTAACGCGGATCAAGGGAAGCCACCATCAGTT
TAAGCACCCGAACTCCCGTCTGGTGATCACCGTTCCACATCCGCGAAAGGATCTGAAAGCGGGGACGCTGCGCCAAATCA
TGAAAGATGCCGGATTGTGCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT143420 WP_000729703.1 NZ_CP046025:3652880-3653140 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT143420 NZ_CP061082:3311009-3311422 [Serratia liquefaciens]
ATGTTCTACCCTGCTTATGTACATTCAGAACCGGACGGCAGCGCCAGCGGCTTCTTTCCCGATGTGCCAGGCTGCTATTT
TGCCGGAGATACCCTGGACAGAGCCTTTGAAGACGCCAGAAGTGCGCTGGATGCCCATTTCGAATTCTTGAGCGAAGACA
ATCAGCCCATTCCCGGCCCACAGGCCGTGACCTTTCATCTGGCACAGAATAACGACAGTCTGAATGGCGGGCAGTGGTTG
TTGGTCAATATTAATATGGATAAGTTTGATGGTCGGGCGGAGCGTATCAATATTACCTTGCCGCACCGGCTGCTGAACCG
CATAGATTCTCTGGTGCAGCAGCATCCCGGTTACGGTAGCCGCAGCGCCTTTTTGGCCGCCGCCGCCCGCAATGAATTAT
TGAAAGCCGGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References