Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3646683..3647377 Replicon chromosome
Accession NZ_CP046025
Organism Escherichia coli strain C439

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag GJH08_RS17815 Protein ID WP_001263489.1
Coordinates 3646683..3647081 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag GJH08_RS17820 Protein ID WP_000554758.1
Coordinates 3647084..3647377 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3642271..3642351 - 81 NuclAT_12 - -
- 3642271..3642351 - 81 NuclAT_12 - -
- 3642271..3642351 - 81 NuclAT_12 - -
- 3642271..3642351 - 81 NuclAT_12 - -
GJH08_RS17790 3642947..3643405 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
GJH08_RS17795 3643666..3645123 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
GJH08_RS17800 3645180..3645701 - 522 Protein_3479 peptide chain release factor H -
GJH08_RS17805 3645697..3645903 - 207 Protein_3480 RtcB family protein -
GJH08_RS17810 3646221..3646673 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
GJH08_RS17815 3646683..3647081 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
GJH08_RS17820 3647084..3647377 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
GJH08_RS17825 3647429..3648484 - 1056 WP_001226164.1 DNA polymerase IV -
GJH08_RS17830 3648555..3649340 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
GJH08_RS17835 3649312..3651024 + 1713 Protein_3486 flagellar biosynthesis protein FlhA -
GJH08_RS17840 3651248..3651745 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T143419 WP_001263489.1 NZ_CP046025:c3647081-3646683 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T143419 NZ_CP061082:2116564-2116660 [Serratia liquefaciens]
AACAAGCCTTGCACAAAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTAGACCGAATATAGGAATC
GTATTCGGTCTTTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT143419 WP_000554758.1 NZ_CP046025:c3647377-3647084 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT143419 NZ_CP061082:c2116665-2116542 [Serratia liquefaciens]
AACACAAAAAAAGACCGAATACGATTCCTATATTCGGTCTAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTTTGTGCAAGGCTTGTTCAGCCATGCACTTTAAGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References