Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1794526..1795028 Replicon chromosome
Accession NZ_CP046025
Organism Escherichia coli strain C439

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag GJH08_RS08465 Protein ID WP_000767829.1
Coordinates 1794774..1795028 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag GJH08_RS08460 Protein ID WP_001259255.1
Coordinates 1794526..1794777 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJH08_RS08435 1789704..1790771 - 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
GJH08_RS08440 1790771..1791841 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
GJH08_RS08445 1791838..1793142 - 1305 WP_000009594.1 histidinol dehydrogenase -
GJH08_RS08450 1793148..1794047 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
GJH08_RS08455 1794193..1794243 - 51 WP_001364200.1 his operon leader peptide -
GJH08_RS08460 1794526..1794777 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
GJH08_RS08465 1794774..1795028 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
GJH08_RS08470 1795111..1795935 + 825 WP_000754737.1 SDR family oxidoreductase -
GJH08_RS08475 1795981..1796910 + 930 WP_000803351.1 LysR family transcriptional regulator -
GJH08_RS08480 1797125..1797187 + 63 WP_010723108.1 membrane protein YoeI -
GJH08_RS08485 1797177..1798535 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
GJH08_RS08490 1798714..1799772 + 1059 WP_000492339.1 transport protein YeeE -
GJH08_RS08495 1799786..1800013 + 228 WP_000234896.1 sulfurtransferase TusA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T143404 WP_000767829.1 NZ_CP046025:1794774-1795028 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T143404 NZ_CP061081:c1758642-1758373 [Marinomonas arctica]
ATGTATCAGCTTGTATATTCAACACAATTCAAAAAAGATTTTAAGAAAATCACCAAAATGCCCATTGCCGACATCATTGA
AGTTGGGAATATCATTTCCCGCCTACAGCGCGGAGAAAGCTTAGATACTAAGCACGTAAATCATCCACTAACAGGAAATT
GGCTCGGATTCAAAGACTGCCATATAAAACCGGATTTGGTTCTTATTTACAGAGTAATAGATAACGCCTTACAACTGGCT
AGAATTGGATCTCATAGTGACGTTTTTTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT143404 WP_001259255.1 NZ_CP046025:1794526-1794777 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT143404 NZ_CP061081:c1758915-1758649 [Marinomonas arctica]
ATGAAGAACGAAATGCTCAGTACTCGTATAGATCACGAAACTAAAGCCGCGTTTACACACATCTGTGATGAAGTCGGTTT
AAGCCCTTCACAAGCCATTAAGCTGTTTGCGAAAGCCGTTATCAACCACGGCGGAATTCCGTTTGATATAAAAATGCGTC
AACCAAATACAGACACCATCGCCGCAATGAAAGAGCTGAATCAAGGCCAAGGACATAAGGCCGAATCCGTCAGCAGCATG
TTTGAGGAATTAACAGAGGCAAGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References