Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3643967..3644508 Replicon chromosome
Accession NZ_CP046009
Organism Escherichia coli strain 1919D62

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag GJD96_RS19140 Protein ID WP_000615979.1
Coordinates 3644230..3644508 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag GJD96_RS19135 Protein ID WP_000729704.1
Coordinates 3643967..3644227 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJD96_RS19110 3639749..3640535 - 787 Protein_3421 putative lateral flagellar export/assembly protein LafU -
GJD96_RS19115 3640507..3642219 + 1713 Protein_3422 flagellar biosynthesis protein FlhA -
GJD96_RS19120 3642324..3642602 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
GJD96_RS19125 3642595..3642951 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
GJD96_RS19130 3643008..3643781 - 774 WP_001353765.1 C40 family peptidase -
GJD96_RS19135 3643967..3644227 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
GJD96_RS19140 3644230..3644508 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
GJD96_RS19145 3644664..3645404 + 741 WP_001225679.1 murein L,D-transpeptidase -
GJD96_RS19150 3645375..3646142 - 768 WP_000333379.1 class II glutamine amidotransferase -
GJD96_RS19155 3646348..3646926 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3642324..3654300 11976


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T143361 WP_000615979.1 NZ_CP046009:3644230-3644508 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T143361 NZ_CP061062:c4040864-4040580 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT143361 WP_000729704.1 NZ_CP046009:3643967-3644227 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT143361 NZ_CP061062:c4041096-4040854 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References