Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1828377..1828879 Replicon chromosome
Accession NZ_CP046009
Organism Escherichia coli strain 1919D62

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag GJD96_RS10350 Protein ID WP_000767829.1
Coordinates 1828625..1828879 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag GJD96_RS10345 Protein ID WP_001259255.1
Coordinates 1828377..1828628 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJD96_RS10320 1823555..1824622 - 1068 WP_032248774.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
GJD96_RS10325 1824622..1825692 - 1071 WP_032248775.1 histidinol-phosphate transaminase -
GJD96_RS10330 1825689..1826993 - 1305 WP_001572810.1 histidinol dehydrogenase -
GJD96_RS10335 1826999..1827898 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
GJD96_RS10340 1828044..1828094 - 51 WP_001364200.1 his operon leader peptide -
GJD96_RS10345 1828377..1828628 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
GJD96_RS10350 1828625..1828879 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
GJD96_RS10355 1828962..1829786 + 825 WP_000754737.1 SDR family oxidoreductase -
GJD96_RS10360 1829832..1830761 + 930 WP_000803360.1 LysR family transcriptional regulator -
GJD96_RS10365 1830976..1831038 + 63 WP_010723108.1 membrane protein YoeI -
GJD96_RS10370 1831028..1832386 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
GJD96_RS10375 1832565..1833623 + 1059 WP_154167001.1 transport protein YeeE -
GJD96_RS10380 1833637..1833864 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T143348 WP_000767829.1 NZ_CP046009:1828625-1828879 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T143348 NZ_CP061053:2056220-2056322 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT143348 WP_001259255.1 NZ_CP046009:1828377-1828628 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT143348 NZ_CP061053:c2056329-2056184 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References