Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3691660..3692201 Replicon chromosome
Accession NZ_CP046006
Organism Escherichia coli strain 1919D3

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag GJD95_RS19235 Protein ID WP_000615976.1
Coordinates 3691923..3692201 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag GJD95_RS19230 Protein ID WP_000729704.1
Coordinates 3691660..3691920 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJD95_RS19215 3687719..3689812 + 2094 WP_154166460.1 flagellar biosynthesis protein FlhA -
GJD95_RS19220 3690028..3690525 - 498 WP_000006261.1 REP-associated tyrosine transposase RayT -
GJD95_RS19225 3690701..3691474 - 774 WP_000543899.1 C40 family peptidase -
GJD95_RS19230 3691660..3691920 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
GJD95_RS19235 3691923..3692201 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
GJD95_RS19240 3692357..3693097 + 741 WP_001225679.1 murein L,D-transpeptidase -
GJD95_RS19245 3693068..3693835 - 768 WP_000333380.1 class II glutamine amidotransferase -
GJD95_RS19250 3694041..3694619 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T143340 WP_000615976.1 NZ_CP046006:3691923-3692201 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T143340 NZ_CP061043:c1407-1141 [Staphylococcus warneri]
ATGGCTAAGTTAAATATTACATTTTCACCCCAAGCATTTGAAGATTATGAGTATTTTCAACAGAATGATAAAAAGCTGGT
AAAAAAGATTAACGACTTGCTTAAAAGTATTAGTAGAGATGGTGTTTTAAAAGGCATAGGCAAACCCGAAAAGTTAAGAA
CGAATTTCTCTGGGTATTATAGTCGACGTATTAATCATGAACATAGATTGGTTTATACAGTTGATGATTATCATATCAAG
ATAGCTTCGTGTAGATATCATTATTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT143340 WP_000729704.1 NZ_CP046006:3691660-3691920 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT143340 NZ_CP061043:c1658-1407 [Staphylococcus warneri]
ATGTTAGTTAAAAACTACTCTAACGCAAGACAAAATTTAAAAGCGCTGATGACACGAGTGAATGATGATAGTGATGTAGT
TACAGTCACATCAACTGATAATAAAAATGTAGTGATGATGTCTGAGTCTGACTACAATTCAATGATGGAAACACTTTATT
TACAACAGAACCCAAAAAATGCTGAACATATAGCTCAATCAATTGAAGATTTAGAACGTGGCAATACAATTACGAAAGAA
ATTGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References