Detailed information of TA system TA143339

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3650178..3650872
• Replicon: chromosome
• Accession: NZ_CP046006
• Organism: Escherichia coli strain 1919D3

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: GJD95_RS18995
• Protein ID: WP_001263493.1
• Coordinates: 3650178..3650576 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: GJD95_RS19000
• Protein ID: WP_000554757.1
• Coordinates: 3650579..3650872 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJD95_RS18965	3645178..3646422	-	1245	WP_124884798.1	esterase FrsA	-
-	3645838..3645918	-	81	NuclAT_12	-	-
-	3645838..3645918	-	81	NuclAT_12	-	-
-	3645838..3645918	-	81	NuclAT_12	-	-
-	3645838..3645918	-	81	NuclAT_12	-	-
GJD95_RS18970	3646514..3646972	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
GJD95_RS18975	3647233..3648690	+	1458	WP_001293016.1	cytosol nonspecific dipeptidase	-
GJD95_RS18980	3648747..3649268	-	522	Protein_3383	peptide chain release factor H	-
GJD95_RS18985	3649267..3649470	-	204	Protein_3384	RNA ligase RtcB family protein	-
GJD95_RS18990	3649716..3650168	-	453	WP_001059855.1	GNAT family N-acetyltransferase	-
GJD95_RS18995	3650178..3650576	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
GJD95_RS19000	3650579..3650872	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
GJD95_RS19005	3650924..3651979	-	1056	WP_040079023.1	DNA polymerase IV	-
GJD95_RS19010	3652050..3652973	-	924	WP_154166458.1	putative lateral flagellar export/assembly protein LafU	-
GJD95_RS19015	3652976..3653839	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
GJD95_RS19020	3653852..3654571	-	720	WP_000938740.1	FliA/WhiG family RNA polymerase sigma factor	-
GJD95_RS19025	3654591..3655058	-	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	3600591..3650872	50281

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T143339 WP_001263493.1 NZ_CP046006:c3650576-3650178 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T143339 NZ_CP061041:c1924713-1924528 [Staphylococcus warneri]
ATGAGCTTGCATTTTACAATTCTTTTTTGGCTTTCACTTATATTTATCGTAGCTGGTGCGATTATTCTAGCTATCATGCT
TAAAACTAAAAAAGAATCTAAGAAAGAATCTTATCTTGGTTTCACCATTGTATTCTTCATTTTTGGATTAGCTATGTTGA
TATATACATTATTATTCGGACTCTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT143339 WP_000554757.1 NZ_CP046006:c3650872-3650579 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT143339 NZ_CP061041:c1924990-1924733 [Staphylococcus warneri]
ATGAAACTATACATTATACATGCAATCAATATTATTTTAACGATATTATTTATTATTTTTAATGTAATTATTACTTATAA
TGCAAACCTAGACGATACCTTATGGTTAGTGCCTGGATTAATTGTATGCGGTTTGATTATGATGATTAGTTTTGCTATTG
CTATAACTAAAAAGGACTTGTTAAGTGAAGTTCTTTTCTTTATTAATATTATTCTTACACTGTACTACATTTATCCAATT
TTTTATGATTTTCTTTAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6