Detailed information of TA system TA143316

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1218..1443
• Replicon: plasmid p1919D3-2
• Accession: NZ_CP046005
• Organism: Escherichia coli strain 1919D3

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: GJD95_RS01250
• Protein ID: WP_000887491.1
• Coordinates: 1231..1443 (+)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1218..1276 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJD95_RS01245	861..1106	+	246	WP_001369095.1	hypothetical protein	-
-	1218..1276	-	59	-	-	Antitoxin
GJD95_RS01250	1231..1443	+	213	WP_000887491.1	type I toxin-antitoxin system Hok family toxin	Toxin
GJD95_RS01255	1562..2071	-	510	WP_000509939.1	hypothetical protein	-
GJD95_RS01260	2083..2664	-	582	WP_000035301.1	hypothetical protein	-
GJD95_RS01265	2700..3515	-	816	WP_000041772.1	hypothetical protein	-
GJD95_RS01270	3525..5114	-	1590	WP_000085146.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..95543	95543

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7857.40 Da
• Isoelectric Point: 8.2634

>T143316 WP_000887491.1 NZ_CP046005:1231-1443 [Escherichia coli]
MLDTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 213 bp

>T143316 NZ_CP061032:591472-591765 [Corynebacterium sp. zg-917]
ATGAAGTATCGGCTTAGGCGCCAAGCAGAATCTGACCTTGTAGAGATTTGGAACTACACGGCCGTTCGTTGGGGAATGTC
CCAGGCTGAGCGCTATGTTGGCCAAGTTCTGTATATGCTCGACATGCTTGGAGGAAACCCGCGGCTCGGAAGCTCCGCAA
GCGAAGTTCGCTCTGGCTACCGAAAGAAAGCAGTCGGGAGCCACATCATCTTCTACCGGGAAGACGACGGTGTCATCGAT
GTGGTCCGAATCTTGCACCAACGCATGGATCTACAACACCACCTGAATAGCTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT143316 NZ_CP046005:c1276-1218 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTCTAGCATGAAATTGGCCTCA

Structures

Antitoxin