Detailed information of TA system TA143313

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3738030..3738724
• Replicon: chromosome
• Accession: NZ_CP046003
• Organism: Escherichia coli strain 1916D6

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: GJD94_RS18935
• Protein ID: WP_001263493.1
• Coordinates: 3738030..3738428 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: GJD94_RS18940
• Protein ID: WP_000554757.1
• Coordinates: 3738431..3738724 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJD94_RS18905	3733589..3734047	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
GJD94_RS18910	3734308..3735765	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
GJD94_RS18915	3735822..3736343	-	522	Protein_3556	peptide chain release factor H	-
GJD94_RS18920	3736342..3736545	-	204	Protein_3557	RNA ligase RtcB family protein	-
GJD94_RS18925	3736791..3737243	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
GJD94_RS18930	3737317..3738014	+	698	Protein_3559	IS1 family transposase	-
GJD94_RS18935	3738030..3738428	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
GJD94_RS18940	3738431..3738724	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
GJD94_RS18945	3738776..3739831	-	1056	WP_001226164.1	DNA polymerase IV	-
GJD94_RS18950	3739902..3740687	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
GJD94_RS18955	3740659..3742371	+	1713	Protein_3564	flagellar biosynthesis protein FlhA	-
GJD94_RS18960	3742595..3743092	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3737721..3738014	293

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T143313 WP_001263493.1 NZ_CP046003:c3738428-3738030 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T143313 NZ_CP061030:c333276-333055 [Staphylococcus pseudintermedius]
ATGGAAAATGGTTATGTTAAAGTAACACAACTGACAACTGAAGCACAGCAACAACAGCTATCTGATCGTTTGCTTCGTTT
AATTGGTGTCGAGTCTGTTGCTATTGATGTGGCAACACAATCGTTGACGCTCACTTATGACACGCCAGCAAATTTGAACA
CGATAGAAAAAGAAATTTATGATGCAGGATATCCTGTTATCCAATCACATAAAGGAGTGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT143313 WP_000554757.1 NZ_CP046003:c3738724-3738431 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT143313 NZ_CP061030:c333576-333289 [Staphylococcus pseudintermedius]
ATGACTGAACAAGCACATCATTCTGAAGAAATTAAACGTAACTTGAAGTCGCGTTTGAATCGTATTGAAGGCCAAGTGAA
AGCAATCAACCGAATGATTGACGAAGATGTCTATTGTGATGACGTGTTGACGCAAATTAGAGCGACACGTTCGGCTTTAA
ACAGTGTGGCGTCGCGTCTGTTAGATCATCATATGAAAGGATGCATCAAGCATAAGATTGACGCGGGACACCAAGACGAA
GCGATGGAAGAGTTATTAGTAACATTTCAAAAACTCATGAAAGATTAG

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6