Detailed information of TA system TA143293

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4624167..4624769
• Replicon: chromosome
• Accession: NZ_CP046000
• Organism: Escherichia coli strain 1916D18

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: GJD92_RS22895
• Protein ID: WP_000897305.1
• Coordinates: 4624458..4624769 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GJD92_RS22890
• Protein ID: WP_000356397.1
• Coordinates: 4624167..4624457 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJD92_RS22865	4620093..4620995	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
GJD92_RS22870	4620992..4621627	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
GJD92_RS22875	4621624..4622553	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
GJD92_RS22880	4622883..4623125	-	243	WP_001086388.1	hypothetical protein	-
GJD92_RS22885	4623344..4623562	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
GJD92_RS22890	4624167..4624457	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
GJD92_RS22895	4624458..4624769	-	312	WP_000897305.1	hypothetical protein	Toxin
GJD92_RS22900	4624998..4625906	+	909	WP_001162704.1	alpha/beta hydrolase	-
GJD92_RS22905	4625970..4626911	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
GJD92_RS22910	4626956..4627393	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
GJD92_RS22915	4627390..4628262	-	873	WP_000920762.1	virulence factor BrkB family protein	-
GJD92_RS22920	4628256..4628855	-	600	WP_001315111.1	glucose-1-phosphatase	-
GJD92_RS22925	4628954..4629739	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T143293 WP_000897305.1 NZ_CP046000:c4624769-4624458 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T143293 NZ_CP060987:c4468479-4468306 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT143293 WP_000356397.1 NZ_CP046000:c4624457-4624167 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT143293 NZ_CP060987:c4468803-4468507 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7