Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 626240..627039 Replicon chromosome
Accession NZ_CP046000
Organism Escherichia coli strain 1916D18

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag GJD92_RS03770 Protein ID WP_000347267.1
Coordinates 626240..626704 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag GJD92_RS03775 Protein ID WP_001307405.1
Coordinates 626704..627039 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJD92_RS03740 621241..621675 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
GJD92_RS03745 621693..622571 - 879 WP_001298758.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
GJD92_RS03750 622561..623340 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
GJD92_RS03755 623351..623824 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
GJD92_RS03760 623847..625127 - 1281 WP_000681947.1 tagatose-bisphosphate aldolase subunit KbaZ -
GJD92_RS03765 625376..626185 + 810 WP_000072180.1 aga operon transcriptional regulator AgaR -
GJD92_RS03770 626240..626704 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
GJD92_RS03775 626704..627039 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
GJD92_RS03780 627188..628759 - 1572 WP_001273741.1 galactarate dehydratase -
GJD92_RS03785 629134..630468 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
GJD92_RS03790 630484..631254 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 626240..639306 13066


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T143272 WP_000347267.1 NZ_CP046000:c626704-626240 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T143272 NZ_CP060983:7110-7358 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTCTGGGGTATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT143272 WP_001307405.1 NZ_CP046000:c627039-626704 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT143272 NZ_CP060983:7355-7702 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References