Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3068976..3069703 Replicon chromosome
Accession NZ_CP045977
Organism Escherichia coli strain INSC1002

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag GI529_RS14835 Protein ID WP_000550189.1
Coordinates 3069389..3069703 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag GI529_RS14830 Protein ID WP_000560266.1
Coordinates 3068976..3069392 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GI529_RS14820 3064136..3066487 + 2352 WP_000695489.1 alpha-glucosidase -
GI529_RS14825 3066913..3068931 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
GI529_RS14830 3068976..3069392 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
GI529_RS14835 3069389..3069703 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
GI529_RS14840 3069987..3071123 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
GI529_RS14845 3071208..3071711 + 504 WP_001300832.1 M48 family metallopeptidase -
GI529_RS14850 3071788..3072480 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
GI529_RS14855 3072559..3073545 + 987 WP_012775974.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T143233 WP_000550189.1 NZ_CP045977:c3069703-3069389 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T143233 NZ_CP060974:2739935-2740042 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT143233 WP_000560266.1 NZ_CP045977:c3069392-3068976 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT143233 NZ_CP060974:c2739888-2739822 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References