Detailed information of TA system TA143211

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 256572..257266
• Replicon: chromosome
• Accession: NZ_CP045977
• Organism: Escherichia coli strain INSC1002

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: GI529_RS01170
• Protein ID: WP_001263493.1
• Coordinates: 256868..257266 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: GI529_RS01165
• Protein ID: WP_000554757.1
• Coordinates: 256572..256865 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GI529_RS01145	252212..252709	+	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-
GI529_RS01150	252925..254637	-	1713	Protein_224	flagellar biosynthesis protein FlhA	-
GI529_RS01155	254609..255394	+	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
GI529_RS01160	255465..256520	+	1056	WP_001226164.1	DNA polymerase IV	-
GI529_RS01165	256572..256865	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
GI529_RS01170	256868..257266	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
GI529_RS01175	257276..257728	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
GI529_RS01180	257974..258177	+	204	Protein_230	RNA ligase RtcB family protein	-
GI529_RS01185	258176..258697	+	522	Protein_231	peptide chain release factor H	-
GI529_RS01190	258754..260211	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
GI529_RS01195	260472..260930	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
GI529_RS01200	261022..262266	+	1245	WP_000189541.1	esterase FrsA	-
-	261526..261606	+	81	NuclAT_13	-	-
-	261526..261606	+	81	NuclAT_13	-	-
-	261526..261606	+	81	NuclAT_13	-	-
-	261526..261606	+	81	NuclAT_13	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T143211 WP_001263493.1 NZ_CP045977:256868-257266 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T143211 NZ_CP060969:3760852-3761130 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT143211 WP_000554757.1 NZ_CP045977:256572-256865 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT143211 NZ_CP060969:3760589-3760849 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTACCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATTAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6