Detailed information of TA system TA143202

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4459464..4460158
• Replicon: chromosome
• Accession: NZ_CP045975
• Organism: Escherichia coli strain AUSMDU00002545

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: GJD97_RS22525
• Protein ID: WP_001263495.1
• Coordinates: 4459464..4459862 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: GJD97_RS22530
• Protein ID: WP_000554757.1
• Coordinates: 4459865..4460158 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4455051..4455131	-	81	NuclAT_12	-	-
-	4455051..4455131	-	81	NuclAT_12	-	-
-	4455051..4455131	-	81	NuclAT_12	-	-
-	4455051..4455131	-	81	NuclAT_12	-	-
GJD97_RS22500	4455727..4456185	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
GJD97_RS22505	4456446..4457903	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
GJD97_RS22510	4457960..4458481	-	522	Protein_4394	peptide chain release factor H	-
GJD97_RS22515	4458480..4458683	-	204	Protein_4395	RNA ligase RtcB family protein	-
GJD97_RS22520	4459002..4459454	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
GJD97_RS22525	4459464..4459862	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
GJD97_RS22530	4459865..4460158	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
GJD97_RS22535	4460210..4461265	-	1056	WP_001226186.1	DNA polymerase IV	-
GJD97_RS22540	4461336..4462121	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
GJD97_RS22545	4462093..4463805	+	1713	Protein_4401	flagellar biosynthesis protein FlhA	-
GJD97_RS22550	4463950..4464447	-	498	Protein_4402	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T143202 WP_001263495.1 NZ_CP045975:c4459862-4459464 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T143202 NZ_CP060969:1854898-1855152 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT143202 WP_000554757.1 NZ_CP045975:c4460158-4459865 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT143202 NZ_CP060969:1854650-1854901 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: S1FJN6