Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) HipBST/HipA(toxin)
Location 2724933..2726176 Replicon chromosome
Accession NZ_CP045974
Organism Legionella pneumophila strain AUSMDU00010536 isolate SBT211

Toxin (Protein)


Gene name HipT Uniprot ID Q5ZSZ6
Locus tag GJD98_RS12215 Protein ID WP_010948074.1
Coordinates 2725238..2726176 (+) Length 313 a.a.

Antitoxin (Protein)


Gene name HipS Uniprot ID Q5ZSZ7
Locus tag GJD98_RS12210 Protein ID WP_010948073.1
Coordinates 2724933..2725241 (+) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJD98_RS12180 2720369..2720734 + 366 WP_010948069.1 BRCT domain-containing protein -
GJD98_RS12185 2721091..2721456 - 366 WP_010948070.1 TraK family protein -
GJD98_RS12190 2721641..2721781 - 141 WP_153802426.1 hypothetical protein -
GJD98_RS12195 2721784..2723535 - 1752 WP_010948071.1 DUF927 domain-containing protein -
GJD98_RS12200 2723601..2723876 - 276 WP_010948072.1 helix-turn-helix domain-containing protein -
GJD98_RS12205 2724709..2724936 + 228 WP_006870829.1 helix-turn-helix transcriptional regulator -
GJD98_RS12210 2724933..2725241 + 309 WP_010948073.1 HipA N-terminal domain-containing protein Antitoxin
GJD98_RS12215 2725238..2726176 + 939 WP_010948074.1 lpg2370 family Dot/Icm T4SS effector Toxin
GJD98_RS12225 2726719..2727333 + 615 WP_010948075.1 hypothetical protein -
GJD98_RS12230 2727489..2727695 - 207 WP_015444115.1 hypothetical protein -
GJD98_RS12235 2728032..2729300 + 1269 WP_010948076.1 lpg2372 family Dot/Icm T4SS effector -
GJD98_RS12240 2729516..2730041 - 526 Protein_2377 hypothetical protein -
GJD98_RS12245 2730041..2730706 - 666 WP_015444114.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2718955..2720130 1175


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(53-276)

Antitoxin

HipA, N-terminal subdomain 1

(5-98)


Sequences

Toxin        


Download         Length: 313 a.a.        Molecular weight: 36077.81 Da        Isoelectric Point: 8.6882

>T143179 WP_010948074.1 NZ_CP045974:2725238-2726176 [Legionella pneumophila]
MKHCPITYEKISDQENYSQRGLHLLSPQLKNLSPLDLSADEQRQEAIARVGKMSVQGVQKKLSAKLKIKEGCFEIVDQYG
QYILKPQSDIYPELPENEAITMTLAKTIGLEVPVHGLVYSKDNSLTYFIKRFDRIGHNKKLALEDFAQLSGEDRHTKYKS
SMEKVIAVIEQFCTFPKIEFVKLFKLTLFNFLVGNEDMHLKNFSLITKDRKISISPAYDLLNSTIAQKNTKEELALPLKG
KKNNLTKSDFLKYFAIEKLGLNQNVIDGIVQEFHQVIPKWQELIGFSFLSQEMQEKYLELLEQRCKRLNFFD

Download         Length: 939 bp

>T143179 NZ_CP060963:2029912-2030015 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11557.38 Da        Isoelectric Point: 4.5994

>AT143179 WP_010948073.1 NZ_CP045974:2724933-2725241 [Legionella pneumophila]
MRKAYVSVSGIKAGILEELQGGTYQFTYFEDYHGAPVSLTMPLKNKVYDFDVFPPFFEGLLPEGIMLEALLRKYKIDKND
YFGQLILVGQDVVGAVTIEEIR

Download         Length: 309 bp

>AT143179 NZ_CP060963:c2030053-2029782 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VKC
PDB 7VKB
PDB 7WCF

Antitoxin

Source ID Structure
PDB 7VKB
AlphaFold DB Q5ZSZ7

References