Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2272194..2273113 Replicon chromosome
Accession NZ_CP045962
Organism Mycobacterium tuberculosis strain AUSMDU00018547

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag GJE03_RS10610 Protein ID WP_003900449.1
Coordinates 2272508..2273113 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag GJE03_RS10605 Protein ID WP_003410124.1
Coordinates 2272194..2272499 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJE03_RS10575 2267205..2268461 - 1257 WP_003902247.1 HNH endonuclease -
GJE03_RS10580 2268815..2269390 + 576 WP_003410100.1 hypothetical protein -
GJE03_RS10585 2269387..2270427 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
GJE03_RS10590 2270669..2271388 + 720 WP_003410108.1 DUF433 domain-containing protein -
GJE03_RS10595 2271378..2271794 + 417 WP_003410114.1 hypothetical protein -
GJE03_RS10600 2271810..2272109 - 300 WP_003410120.1 hypothetical protein -
GJE03_RS10605 2272194..2272499 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
GJE03_RS10610 2272508..2273113 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GJE03_RS10615 2273138..2273497 - 360 WP_003410131.1 hypothetical protein -
GJE03_RS10620 2273657..2274334 - 678 WP_003900450.1 hypothetical protein -
GJE03_RS10625 2274286..2274522 - 237 WP_003410133.1 hypothetical protein -
GJE03_RS10630 2274729..2275625 - 897 WP_003410136.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T143141 WP_003900449.1 NZ_CP045962:c2273113-2272508 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T143141 NZ_CP060950:c4047608-4047501 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT143141 WP_003410124.1 NZ_CP045962:c2272499-2272194 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT143141 NZ_CP060950:4047655-4047721 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References