Detailed information of TA system TA143092

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 82451..82704
• Replicon: plasmid pAUSMDU00010535_02
• Accession: NZ_CP045943
• Organism: Shigella flexneri 2a strain AUSMDU00010535

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: GJE14_RS25575
• Protein ID: WP_001312851.1
• Coordinates: 82555..82704 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	82451..82510 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJE14_RS25540 (78385)	78385..79131	+	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-
GJE14_RS25545 (79186)	79186..79746	+	561	WP_033807966.1	fertility inhibition protein FinO	-
GJE14_RS25550 (79878)	79878..80090	+	213	WP_001301409.1	ANR family transcriptional regulator	-
GJE14_RS25555 (80336)	80336..80797	+	462	WP_001233850.1	thermonuclease family protein	-
GJE14_RS25560 (80843)	80843..81052	+	210	WP_001298565.1	hemolysin expression modulator Hha	-
GJE14_RS25565 (81090)	81090..81680	+	591	WP_033807967.1	DUF2726 domain-containing protein	-
GJE14_RS25570 (81835)	81835..82308	+	474	WP_016240489.1	hypothetical protein	-
- (82451)	82451..82510	-	60	NuclAT_1	-	Antitoxin
- (82451)	82451..82510	-	60	NuclAT_1	-	Antitoxin
- (82451)	82451..82510	-	60	NuclAT_1	-	Antitoxin
- (82451)	82451..82510	-	60	NuclAT_1	-	Antitoxin
GJE14_RS25575 (82555)	82555..82704	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
GJE14_RS25580 (82989)	82989..83237	+	249	WP_000083839.1	replication regulatory protein RepA	-
GJE14_RS26535 (83352)	83352..83536	+	185	Protein_107	protein CopA/IncA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / aadA5 / qacE / sul1 / mph(A) / erm(B)	-	1..83548	83548

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T143092 WP_001312851.1 NZ_CP045943:82555-82704 [Shigella flexneri 2a]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T143092 NZ_CP060939:c26155-25739 [Escherichia coli]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCTGCCCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT143092 NZ_CP045943:c82510-82451 [Shigella flexneri 2a]
AATGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTTGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin