Detailed information of TA system TA143089

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 40992..41261
• Replicon: plasmid pAUSMDU00010535_02
• Accession: NZ_CP045943
• Organism: Shigella flexneri 2a strain AUSMDU00010535

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: GJE14_RS25315
• Protein ID: WP_001372321.1
• Coordinates: 41136..41261 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	40992..41057 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJE14_RS26525	36041..36484	-	444	Protein_46	hypothetical protein	-
GJE14_RS25285	36786..37307	+	522	WP_033807850.1	single-stranded DNA-binding protein	-
GJE14_RS25290	37363..37596	+	234	WP_000005971.1	DUF905 domain-containing protein	-
GJE14_RS25295	37660..39618	+	1959	WP_112324844.1	ParB/RepB/Spo0J family partition protein	-
GJE14_RS25300	39673..40107	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
GJE14_RS25305	40104..40866	+	763	Protein_51	plasmid SOS inhibition protein A	-
-	40835..41059	+	225	NuclAT_0	-	-
-	40835..41059	+	225	NuclAT_0	-	-
-	40835..41059	+	225	NuclAT_0	-	-
-	40835..41059	+	225	NuclAT_0	-	-
GJE14_RS25310	40844..41023	-	180	WP_001334662.1	hypothetical protein	-
-	40992..41057	+	66	-	-	Antitoxin
GJE14_RS26530	41045..41194	+	150	Protein_53	plasmid maintenance protein Mok	-
GJE14_RS25315	41136..41261	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
GJE14_RS25320	41580..41876	-	297	Protein_55	hypothetical protein	-
GJE14_RS25330	42176..42472	+	297	WP_001272251.1	hypothetical protein	-
GJE14_RS25335	42583..43404	+	822	WP_001234445.1	DUF932 domain-containing protein	-
GJE14_RS25340	43701..44291	-	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
GJE14_RS25345	44624..45007	+	384	WP_001151529.1	conjugal transfer relaxosome DNA-binding protein TraM	-
GJE14_RS25350	45199..45846	+	648	WP_000332523.1	transcriptional regulator TraJ family protein	-
GJE14_RS25355	45966..46193	+	228	WP_000589556.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / aadA5 / qacE / sul1 / mph(A) / erm(B)	-	1..83548	83548

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T143089 WP_001372321.1 NZ_CP045943:41136-41261 [Shigella flexneri 2a]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T143089 NZ_CP060938:c4378839-4378441 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT143089 NZ_CP045943:40992-41057 [Shigella flexneri 2a]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Antitoxin