Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3779411..3780105 Replicon chromosome
Accession NZ_CP045941
Organism Shigella flexneri 2a strain AUSMDU00010535

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag GJE14_RS19190 Protein ID WP_001263491.1
Coordinates 3779411..3779809 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag GJE14_RS19195 Protein ID WP_000554759.1
Coordinates 3779812..3780105 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJE14_RS19165 (3774750) 3774750..3775208 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
GJE14_RS19170 (3775470) 3775470..3776927 + 1458 WP_001293030.1 cytosol nonspecific dipeptidase -
GJE14_RS19175 (3776984) 3776984..3777544 - 561 Protein_3752 peptide chain release factor H -
GJE14_RS19180 (3777660) 3777660..3778703 - 1044 WP_005053205.1 RNA ligase RtcB family protein -
GJE14_RS19185 (3778949) 3778949..3779401 - 453 WP_001059860.1 GNAT family N-acetyltransferase -
GJE14_RS19190 (3779411) 3779411..3779809 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
GJE14_RS19195 (3779812) 3779812..3780105 - 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
GJE14_RS19200 (3780157) 3780157..3781212 - 1056 WP_001226172.1 DNA polymerase IV -
GJE14_RS19205 (3781283) 3781283..3782068 - 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
GJE14_RS19210 (3782040) 3782040..3783752 + 1713 Protein_3759 flagellar biosynthesis protein FlhA -
GJE14_RS19215 (3783969) 3783969..3784466 - 498 WP_000006251.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gtrII / gtrB / gtrA 3738287..3786287 48000


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T143083 WP_001263491.1 NZ_CP045941:c3779809-3779411 [Shigella flexneri 2a]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T143083 NZ_CP060938:2710777-2710880 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT143083 WP_000554759.1 NZ_CP045941:c3780105-3779812 [Shigella flexneri 2a]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT143083 NZ_CP060938:c2710918-2710647 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References