143082

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3692065..3692909 Replicon chromosome
Accession NZ_CP045941
Organism Shigella flexneri 2a strain AUSMDU00010535

Toxin (Protein)


Gene name itaT Uniprot ID -
Locus tag GJE14_RS18740 Protein ID WP_014532172.1
Coordinates 3692448..3692909 (+) Length 154 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID A5H0K0
Locus tag GJE14_RS18735 Protein ID WP_005053053.1
Coordinates 3692065..3692376 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJE14_RS18710 (3687536) 3687536..3688033 + 498 Protein_3662 COX aromatic rich motif-containing protein -
GJE14_RS18715 (3688055) 3688055..3690046 + 1992 Protein_3663 cytochrome o ubiquinol oxidase subunit I -
GJE14_RS18720 (3690036) 3690036..3690650 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
GJE14_RS18725 (3690650) 3690650..3690979 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
GJE14_RS18730 (3690991) 3690991..3691881 + 891 WP_000971346.1 heme o synthase -
GJE14_RS18735 (3692065) 3692065..3692376 + 312 WP_005053053.1 DUF1778 domain-containing protein Antitoxin
GJE14_RS18740 (3692448) 3692448..3692909 + 462 WP_014532172.1 GNAT family N-acetyltransferase Toxin
GJE14_RS18750 (3693805) 3693805..3695958 + 2154 WP_005060811.1 SEL1-like repeat protein -
GJE14_RS18755 (3696011) 3696011..3697741 + 1731 WP_000645013.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 3692957..3693460 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-133)

Antitoxin

Vibrio phage ICP1, Orf50

(15-97)


Sequences

Toxin        


Download         Length: 154 a.a.        Molecular weight: 16623.52 Da        Isoelectric Point: 9.9538

>T143082 WP_014532172.1 NZ_CP045941:3692448-3692909 [Shigella flexneri 2a]
MIAVTLLSINLYALRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVMLGGAR
KYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFYARLGFVQLSATPNAFGAVPMFLAIQHILAA

Download         Length: 462 bp

>T143082 NZ_CP060938:2488472-2488726 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11394.07 Da        Isoelectric Point: 10.3388

>AT143082 WP_005053053.1 NZ_CP045941:3692065-3692376 [Shigella flexneri 2a]
MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT143082 NZ_CP060938:2488224-2488475 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A5H0K0

References