Detailed information of TA system TA143078

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2593711..2593936
• Replicon: chromosome
• Accession: NZ_CP045941
• Organism: Shigella flexneri 2a strain AUSMDU00010535

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: GJE14_RS13070
• Protein ID: WP_000813254.1
• Coordinates: 2593711..2593866 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2593878..2593936 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJE14_RS13030	2588837..2589319	-	483	WP_000068107.1	ORF6N domain-containing protein	-
GJE14_RS13040	2590608..2590781	-	174	WP_000504450.1	hypothetical protein	-
GJE14_RS13045	2590934..2591488	-	555	WP_000640143.1	DUF1133 family protein	-
GJE14_RS13050	2591485..2591775	-	291	WP_073828270.1	DUF1364 domain-containing protein	-
GJE14_RS13055	2591775..2592374	-	600	WP_000940329.1	DUF1367 family protein	-
GJE14_RS13060	2592508..2593205	+	698	WP_225620329.1	IS1 family transposase	-
GJE14_RS13070	2593711..2593866	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	2593878..2593936	+	59	-	-	Antitoxin
GJE14_RS13075	2594321..2594686	-	366	WP_000610655.1	DUF551 domain-containing protein	-
GJE14_RS13080	2594686..2595351	-	666	WP_000208062.1	hypothetical protein	-
GJE14_RS13085	2595348..2595713	-	366	WP_001229297.1	HNH endonuclease signature motif containing protein	-
GJE14_RS13090	2595715..2595933	-	219	WP_000256998.1	DUF4014 family protein	-
GJE14_RS13095	2596026..2596382	-	357	WP_005048249.1	hypothetical protein	-
GJE14_RS13100	2596440..2596862	-	423	WP_001118167.1	DUF977 family protein	-
GJE14_RS13105	2596877..2597623	-	747	WP_000788996.1	ATP-binding protein	-
GJE14_RS26240	2597769..2597938	-	170	Protein_2564	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	sitABCD	ipaH9.8	2536254..2606968	70714

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T143078 WP_000813254.1 NZ_CP045941:c2593866-2593711 [Shigella flexneri 2a]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T143078 NZ_CP060938:1372259-1372555 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT143078 NZ_CP045941:2593878-2593936 [Shigella flexneri 2a]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin