Detailed information of TA system TA143067

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1908756..1908981
• Replicon: chromosome
• Accession: NZ_CP045941
• Organism: Shigella flexneri 2a strain AUSMDU00010535

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: GJE14_RS09340
• Protein ID: WP_000813254.1
• Coordinates: 1908826..1908981 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1908756..1908814 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJE14_RS09305	1903933..1905195	-	1263	Protein_1816	tyrosine-type recombinase/integrase	-
GJE14_RS09315	1905533..1906330	-	798	WP_001325918.1	DgsA anti-repressor MtfA	-
GJE14_RS09325	1906541..1907591	-	1051	Protein_1818	tyrosine-type recombinase/integrase	-
GJE14_RS09330	1907591..1907731	-	141	Protein_1819	DUF4224 domain-containing protein	-
GJE14_RS09335	1907758..1908174	+	417	WP_005069274.1	hypothetical protein	-
-	1908756..1908814	-	59	-	-	Antitoxin
GJE14_RS09340	1908826..1908981	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
GJE14_RS09350	1909149..1909427	+	279	WP_011069426.1	hypothetical protein	-
GJE14_RS09355	1909429..1910487	+	1059	WP_001265248.1	DUF968 domain-containing protein	-
GJE14_RS09360	1910488..1910853	+	366	WP_000140020.1	RusA family crossover junction endodeoxyribonuclease	-
GJE14_RS09365	1910850..1911538	+	689	Protein_1825	bacteriophage antitermination protein Q	-
GJE14_RS09390	1912334..1912549	+	216	WP_000839572.1	class II holin family protein	-
GJE14_RS09395	1912648..1913322	+	675	WP_004967157.1	IS66-like element accessory protein TnpA	-
GJE14_RS09400	1913319..1913669	+	351	WP_005049241.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ipaH9.8	1900625..1940831	40206

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T143067 WP_000813254.1 NZ_CP045941:1908826-1908981 [Shigella flexneri 2a]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T143067 NZ_CP060933:4067148-4067426 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT143067 NZ_CP045941:c1908814-1908756 [Shigella flexneri 2a]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin