Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 744796..745523 Replicon chromosome
Accession NZ_CP045941
Organism Shigella flexneri 2a strain AUSMDU00010535

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag GJE14_RS03705 Protein ID WP_000550189.1
Coordinates 744796..745110 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag GJE14_RS03710 Protein ID WP_000560266.1
Coordinates 745107..745523 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJE14_RS03685 (740941) 740941..741939 - 999 WP_005050984.1 Gfo/Idh/MocA family oxidoreductase -
GJE14_RS03690 (742018) 742018..742710 - 693 WP_000942538.1 vancomycin high temperature exclusion protein -
GJE14_RS03695 (742787) 742787..743290 - 504 WP_005050988.1 M48 family metallopeptidase -
GJE14_RS03700 (743375) 743375..744511 + 1137 WP_000018656.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
GJE14_RS03705 (744796) 744796..745110 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
GJE14_RS03710 (745107) 745107..745523 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
GJE14_RS03715 (745568) 745568..747586 - 2019 WP_000121487.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
GJE14_RS03720 (747812) 747812..750163 - 2352 WP_000695506.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T143064 WP_000550189.1 NZ_CP045941:744796-745110 [Shigella flexneri 2a]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T143064 NZ_CP060933:3012455-3012562 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT143064 WP_000560266.1 NZ_CP045941:745107-745523 [Shigella flexneri 2a]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT143064 NZ_CP060933:c3012405-3012342 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTAAACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References