Detailed information of TA system TA143060

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 79890..80143
• Replicon: plasmid pAUSMDU00010534_02
• Accession: NZ_CP045934
• Organism: Shigella sonnei strain AUSMDU00010534

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: GJE15_RS25235
• Protein ID: WP_001312851.1
• Coordinates: 79994..80143 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	79890..79949 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJE15_RS25200 (75824)	75824..76570	+	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-
GJE15_RS25205 (76625)	76625..77185	+	561	WP_033807966.1	fertility inhibition protein FinO	-
GJE15_RS25210 (77317)	77317..77529	+	213	WP_001301409.1	ANR family transcriptional regulator	-
GJE15_RS25215 (77775)	77775..78236	+	462	WP_001233850.1	thermonuclease family protein	-
GJE15_RS25220 (78282)	78282..78491	+	210	WP_001298565.1	hemolysin expression modulator Hha	-
GJE15_RS25225 (78529)	78529..79119	+	591	WP_033807967.1	DUF2726 domain-containing protein	-
GJE15_RS25230 (79274)	79274..79747	+	474	WP_016240489.1	hypothetical protein	-
- (79890)	79890..79949	-	60	NuclAT_1	-	Antitoxin
- (79890)	79890..79949	-	60	NuclAT_1	-	Antitoxin
- (79890)	79890..79949	-	60	NuclAT_1	-	Antitoxin
- (79890)	79890..79949	-	60	NuclAT_1	-	Antitoxin
GJE15_RS25235 (79994)	79994..80143	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
GJE15_RS25240 (80428)	80428..80676	+	249	WP_000083839.1	replication regulatory protein RepA	-
GJE15_RS26350 (80791)	80791..80975	+	185	Protein_104	protein CopA/IncA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / aadA5 / qacE / sul1 / mph(A) / erm(B)	-	1..80987	80987

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T143060 WP_001312851.1 NZ_CP045934:79994-80143 [Shigella sonnei]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T143060 NZ_CP060933:2689708-2691030 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CAGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTAACGTTATTACCCGAAAA
TGAAACCATAACGCGCCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGGCTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTTAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT143060 NZ_CP045934:c79949-79890 [Shigella sonnei]
AATGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTTGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin