Detailed information of TA system TA143057

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 38404..38673
• Replicon: plasmid pAUSMDU00010534_02
• Accession: NZ_CP045934
• Organism: Shigella sonnei strain AUSMDU00010534

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: GJE15_RS24975
• Protein ID: WP_001372321.1
• Coordinates: 38548..38673 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	38404..38469 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJE15_RS26340	33468..33899	-	432	Protein_43	hypothetical protein	-
GJE15_RS24945	34198..34719	+	522	WP_033807850.1	single-stranded DNA-binding protein	-
GJE15_RS24950	34775..35008	+	234	WP_000005971.1	DUF905 domain-containing protein	-
GJE15_RS24955	35072..37030	+	1959	WP_112324844.1	ParB/RepB/Spo0J family partition protein	-
GJE15_RS24960	37085..37519	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
GJE15_RS24965	37516..38278	+	763	Protein_48	plasmid SOS inhibition protein A	-
-	38247..38471	+	225	NuclAT_0	-	-
-	38247..38471	+	225	NuclAT_0	-	-
-	38247..38471	+	225	NuclAT_0	-	-
-	38247..38471	+	225	NuclAT_0	-	-
GJE15_RS24970	38256..38435	-	180	WP_001334662.1	hypothetical protein	-
-	38404..38469	+	66	-	-	Antitoxin
GJE15_RS26345	38457..38606	+	150	Protein_50	plasmid maintenance protein Mok	-
GJE15_RS24975	38548..38673	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
GJE15_RS24980	38992..39288	-	297	Protein_52	hypothetical protein	-
GJE15_RS24990	39588..39884	+	297	WP_001272251.1	hypothetical protein	-
GJE15_RS24995	39995..40816	+	822	WP_001234445.1	DUF932 domain-containing protein	-
GJE15_RS25000	41113..41703	-	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
GJE15_RS25005	42036..42419	+	384	WP_001151529.1	conjugal transfer relaxosome DNA-binding protein TraM	-
GJE15_RS25010	42611..43258	+	648	WP_000332523.1	transcriptional regulator TraJ family protein	-
GJE15_RS25015	43378..43605	+	228	WP_000589556.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / aadA5 / qacE / sul1 / mph(A) / erm(B)	-	1..80987	80987

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T143057 WP_001372321.1 NZ_CP045934:38548-38673 [Shigella sonnei]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T143057 NZ_CP060933:2157521-2157772 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT143057 NZ_CP045934:38404-38469 [Shigella sonnei]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Antitoxin