Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3535287..3535828 Replicon chromosome
Accession NZ_CP045932
Organism Shigella sonnei strain AUSMDU00010534

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag GJE15_RS17905 Protein ID WP_000615976.1
Coordinates 3535550..3535828 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag GJE15_RS17900 Protein ID WP_000729704.1
Coordinates 3535287..3535547 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJE15_RS17875 (3530756) 3530756..3532468 + 1713 Protein_3555 flagellar biosynthesis protein FlhA -
GJE15_RS17880 (3532591) 3532591..3533088 - 498 WP_000006258.1 REP-associated tyrosine transposase RayT -
GJE15_RS17885 (3533159) 3533159..3533287 - 129 Protein_3557 ISAs1 family transposase -
GJE15_RS17890 (3533342) 3533342..3534039 + 698 WP_094096600.1 IS1-like element IS1A family transposase -
GJE15_RS17895 (3534328) 3534328..3535101 - 774 WP_127148796.1 C40 family peptidase -
GJE15_RS17900 (3535287) 3535287..3535547 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
GJE15_RS17905 (3535550) 3535550..3535828 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
GJE15_RS17910 (3535984) 3535984..3536724 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
GJE15_RS17915 (3536695) 3536695..3537462 - 768 WP_000333380.1 class II glutamine amidotransferase -
GJE15_RS17920 (3537668) 3537668..3538246 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3533715..3546689 12974


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T143055 WP_000615976.1 NZ_CP045932:3535550-3535828 [Shigella sonnei]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T143055 NZ_CP060933:1324000-1324335 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT143055 WP_000729704.1 NZ_CP045932:3535287-3535547 [Shigella sonnei]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT143055 NZ_CP060933:1323752-1324000 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References