Detailed information of TA system TA143042

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 820905..821632
• Replicon: chromosome
• Accession: NZ_CP045932
• Organism: Shigella sonnei strain AUSMDU00010534

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: GJE15_RS04145
• Protein ID: WP_000547563.1
• Coordinates: 820905..821216 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GJE15_RS04150
• Protein ID: WP_000126296.1
• Coordinates: 821213..821632 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJE15_RS04115 (816059)	816059..817756	+	1698	WP_001288143.1	formate hydrogenlyase subunit HycE	-
GJE15_RS04120 (817766)	817766..818308	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
GJE15_RS04125 (818308)	818308..819075	+	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
GJE15_RS04130 (819072)	819072..819482	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
GJE15_RS04135 (819475)	819475..819945	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
GJE15_RS04140 (819970)	819970..820743	+	774	WP_001026459.1	hypothetical protein	-
GJE15_RS04145 (820905)	820905..821216	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
GJE15_RS04150 (821213)	821213..821632	+	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
GJE15_RS04155 (821746)	821746..823170	-	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
GJE15_RS04160 (823179)	823179..824636	-	1458	WP_001107890.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
GJE15_RS04165 (824896)	824896..825906	+	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
GJE15_RS04170 (826054)	826054..826581	+	528	WP_001078766.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T143042 WP_000547563.1 NZ_CP045932:820905-821216 [Shigella sonnei]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T143042 NZ_CP060928:3649235-3649522 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT143042 WP_000126296.1 NZ_CP045932:821213-821632 [Shigella sonnei]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT143042 NZ_CP060928:3649509-3649811 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22