Detailed information of TA system TA143032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 761606..762037
• Replicon: chromosome
• Accession: NZ_CP045929
• Organism: Saccharopolyspora coralli strain E2A

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GIY23_RS03625
• Protein ID: WP_154075362.1
• Coordinates: 761606..761725 (+)
• Length: 40 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GIY23_RS03630
• Protein ID: WP_154075363.1
• Coordinates: 761738..762037 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GIY23_RS03600	756821..757822	+	1002	WP_154075357.1	dipeptide ABC transporter ATP-binding protein	-
GIY23_RS03605	757898..758206	-	309	WP_154075358.1	hypothetical protein	-
GIY23_RS03610	758203..759066	-	864	WP_154075359.1	thermonuclease family protein	-
GIY23_RS03615	759240..760718	-	1479	WP_154075360.1	peptidase S10	-
GIY23_RS03620	760811..761581	+	771	WP_154075361.1	uracil-DNA glycosylase	-
GIY23_RS03625	761606..761725	+	120	WP_154075362.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GIY23_RS03630	761738..762037	+	300	WP_154075363.1	HigA family addiction module antidote protein	Antitoxin
GIY23_RS03635	762034..762393	+	360	WP_154075364.1	hypothetical protein	-
GIY23_RS03640	762432..762518	+	87	Protein_714	uracil-DNA glycosylase	-
GIY23_RS03645	762642..762809	+	168	WP_154075365.1	hypothetical protein	-
GIY23_RS03650	763203..763406	+	204	WP_187352012.1	ribbon-helix-helix protein, CopG family	-
GIY23_RS03655	763403..763780	+	378	WP_154075366.1	type II toxin-antitoxin system death-on-curing family toxin	-
GIY23_RS03660	763797..764606	-	810	WP_154075367.1	metalloregulator ArsR/SmtB family transcription factor	-
GIY23_RS03665	764767..765051	+	285	WP_154075368.1	hypothetical protein	-
GIY23_RS03670	765048..765452	+	405	WP_154075369.1	type II toxin-antitoxin system VapC family toxin	-
GIY23_RS03675	765597..766313	+	717	WP_187352013.1	GNAT family N-acetyltransferase	-
GIY23_RS03680	766405..766824	+	420	WP_154075371.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-39)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-97)

Sequences

Toxin

Protein

• Length: 40 a.a.
• Molecular weight: 4654.17 Da
• Isoelectric Point: 4.6642

>T143032 WP_154075362.1 NZ_CP045929:761606-761725 [Saccharopolyspora coralli]
MQLNGDRAGQYSIRINQQWRICFEWISSGPENVEIVDYH

Nucleotide

• Length: 120 bp

>T143032 NZ_CP060928:1174601-1174975 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11231.82 Da
• Isoelectric Point: 6.4839

>AT143032 WP_154075363.1 NZ_CP045929:761738-762037 [Saccharopolyspora coralli]
MSDEFVMPPVHPGEVLHEEYLEPLGVTQHRLAVAIGVPPRRINEIVHGKRGISADTALRLARFFGTSERFWLNLQSRYDL
ERERGALADTLDRIQPLSA

Nucleotide

• Length: 300 bp

>AT143032 NZ_CP060928:1174144-1174512 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA