Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-VapI
Location 875929..876524 Replicon chromosome
Accession NZ_CP045920
Organism Pseudactinotalea sp. HY158

Toxin (Protein)


Gene name graT Uniprot ID -
Locus tag GCE65_RS03885 Protein ID WP_153877445.1
Coordinates 875929..876210 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag GCE65_RS03890 Protein ID WP_152817523.1
Coordinates 876225..876524 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GCE65_RS03840 871199..872056 - 858 WP_153877439.1 alpha/beta hydrolase -
GCE65_RS03845 872112..872501 + 390 WP_194928820.1 MerR family transcriptional regulator -
GCE65_RS03850 872723..872944 + 222 WP_153877441.1 hypothetical protein -
GCE65_RS03855 872935..873354 + 420 WP_153877442.1 PIN domain nuclease -
GCE65_RS03860 873564..873797 + 234 WP_152817521.1 toxin-antitoxin system -
GCE65_RS03865 873794..874219 + 426 WP_153877443.1 type II toxin-antitoxin system VapC family toxin -
GCE65_RS03870 874216..874732 + 517 Protein_758 GNAT family N-acetyltransferase -
GCE65_RS03875 874733..875239 - 507 WP_153877444.1 SgcJ/EcaC family oxidoreductase -
GCE65_RS03880 875303..875791 + 489 WP_152817522.1 MarR family transcriptional regulator -
GCE65_RS03885 875929..876210 + 282 WP_153877445.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GCE65_RS03890 876225..876524 + 300 WP_152817523.1 HigA family addiction module antidote protein Antitoxin
GCE65_RS03895 876568..876843 + 276 WP_152817524.1 hypothetical protein -
GCE65_RS03900 876822..877352 + 531 WP_194928821.1 hypothetical protein -
GCE65_RS03905 877426..877722 + 297 WP_153877446.1 type II toxin-antitoxin system RelE/ParE family toxin -
GCE65_RS03910 877827..878087 + 261 WP_153877447.1 type II toxin-antitoxin system prevent-host-death family antitoxin -
GCE65_RS03915 878088..878342 + 255 WP_153877448.1 Txe/YoeB family addiction module toxin -
GCE65_RS03920 878439..878768 + 330 WP_152817526.1 nucleotidyltransferase domain-containing protein -
GCE65_RS03925 878765..879100 + 336 WP_153877449.1 DUF86 domain-containing protein -
GCE65_RS16225 879303..879536 + 234 WP_194928898.1 hypothetical protein -
GCE65_RS03935 879816..880184 + 369 WP_153877450.1 carboxymuconolactone decarboxylase family protein -
GCE65_RS03940 880278..880637 + 360 WP_153877451.1 metalloregulator ArsR/SmtB family transcription factor -
GCE65_RS03945 880634..881380 + 747 WP_153877452.1 cation transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 821332..986994 165662


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-93)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(3-96)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10950.42 Da        Isoelectric Point: 10.4333

>T142997 WP_153877445.1 NZ_CP045920:875929-876210 [Pseudactinotalea sp. HY158]
VIRSFADRDTERIWRREPAKRLDPRIHKTANRKLHLRDAATVLDALRVPPGNRLEALKGDRLGQHSIRINDQWRICFRWT
DAGAEDVEVVDYH

Download         Length: 282 bp

>T142997 NZ_CP060919:c4685230-4685057 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11103.87 Da        Isoelectric Point: 7.2017

>AT142997 WP_152817523.1 NZ_CP045920:876225-876524 [Pseudactinotalea sp. HY158]
MSEKLYPPIHPGEVLMEDFIEGFGITQNKLAVAIGVPPRRINEIVHGKRAITADTALRLGRYFAMDPQFWLNLQTRYELE
VAQSRVADQVAAIAPLRTA

Download         Length: 300 bp

>AT142997 NZ_CP060919:c4685554-4685258 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References