Detailed information of TA system TA142975

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2884656..2885284
• Replicon: chromosome
• Accession: NZ_CP045913
• Organism: Serratia proteamaculans strain 336X

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GHV41_RS13920
• Protein ID: WP_153858909.1
• Coordinates: 2884994..2885284 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GHV41_RS13915
• Protein ID: WP_153858908.1
• Coordinates: 2884656..2884979 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHV41_RS13895	2881151..2882125	-	975	WP_167519985.1	TerC family protein	-
GHV41_RS13900	2882387..2883118	-	732	WP_153858906.1	glutamine amidotransferase	-
GHV41_RS13905	2883120..2884100	-	981	WP_153858907.1	Gfo/Idh/MocA family oxidoreductase	-
GHV41_RS13910	2884151..2884663	-	513	WP_012147012.1	M48 family metallopeptidase	-
GHV41_RS13915	2884656..2884979	-	324	WP_153858908.1	HigA family addiction module antidote protein	Antitoxin
GHV41_RS13920	2884994..2885284	-	291	WP_153858909.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GHV41_RS13925	2885515..2886654	+	1140	WP_153858910.1	23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG	-
GHV41_RS13930	2886693..2888714	-	2022	WP_153858911.1	NADPH-dependent 2,4-dienoyl-CoA reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (24-71)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11552.05 Da
• Isoelectric Point: 8.8895

>T142975 WP_153858909.1 NZ_CP045913:c2885284-2884994 [Serratia proteamaculans]
MIPSFRDKYLAQFYQEGRHDRLIPSTIERQLARKLDMLAAAEKECDLHNPTGNYYKRLSGTFQGWSSMRVNIQWRLMFRW
RNDAAEQIYQDPHQDT

Nucleotide

• Length: 291 bp

>T142975 NZ_CP060919:c37377-37066 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12214.94 Da
• Isoelectric Point: 6.7197

>AT142975 WP_153858908.1 NZ_CP045913:c2884979-2884656 [Serratia proteamaculans]
MLETTSRKPTTVGEVLQEEYLTPLGIGQEDLAKVLGVTRATVNRLCNGHRRLSVVEANLFADLFETTADFWLNLQAAHDR
WEARQANMQRERLRPIMEILGRNITHA

Nucleotide

• Length: 324 bp

>AT142975 NZ_CP060919:c37065-36775 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG