Detailed information of TA system TA142959

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4774964..4775553
• Replicon: chromosome
• Accession: NZ_CP045912
• Organism: Xanthomonas oryzae pv. oryzicola strain 0-9

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A0C5V8F2
• Locus tag: GHV42_RS21640
• Protein ID: WP_024712137.1
• Coordinates: 4774964..4775245 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GHV42_RS21645
• Protein ID: WP_011260746.1
• Coordinates: 4775263..4775553 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHV42_RS21635	4772608..4774869	+	2262	WP_153817168.1	exodeoxyribonuclease V subunit alpha	-
GHV42_RS21640	4774964..4775245	+	282	WP_024712137.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GHV42_RS21645	4775263..4775553	+	291	WP_011260746.1	HigA family addiction module antidote protein	Antitoxin
GHV42_RS21650	4776491..4777885	+	1395	WP_149621925.1	nucleoside hydrolase	-
GHV42_RS21660	4778336..4778668	-	333	WP_011260743.1	hypothetical protein	-
GHV42_RS21665	4779004..4779228	+	225	WP_044751358.1	hypothetical protein	-
GHV42_RS21670	4779366..4779800	+	435	WP_071162385.1	PAAR domain-containing protein	-
GHV42_RS21675	4779803..4780279	+	477	WP_024712141.1	DUF4123 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11125.91 Da
• Isoelectric Point: 10.5318

>T142959 WP_024712137.1 NZ_CP045912:4774964-4775245 [Xanthomonas oryzae pv. oryzicola]
MIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYNIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T142959 NZ_CP060914:2161457-2161708 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT142959 WP_011260746.1 NZ_CP045912:4775263-4775553 [Xanthomonas oryzae pv. oryzicola]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT142959 NZ_CP060914:2161705-2161959 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A0C5V8F2