Detailed information of TA system TA142867

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1588046..1588417
• Replicon: chromosome
• Accession: NZ_CP045863
• Organism: Escherichia coli O157:H7 strain ATCC 43890

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: GIX96_RS07875
• Protein ID: WP_001443846.1
• Coordinates: 1588268..1588417 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1588046..1588224 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GIX96_RS07845	1583798..1583968	+	171	WP_001625136.1	hypothetical protein	-
GIX96_RS07850	1584001..1585374	+	1374	WP_000123746.1	ATP-dependent RNA helicase DbpA	-
GIX96_RS07855	1585503..1586438	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
GIX96_RS07860	1586490..1587725	-	1236	WP_000040839.1	site-specific integrase	-
GIX96_RS07865	1587727..1587942	-	216	WP_000079604.1	excisionase XisR	-
GIX96_RS07870	1588042..1588230	-	189	WP_001302840.1	DUF1187 family protein	-
-	1588046..1588224	+	179	NuclAT_0	-	Antitoxin
-	1588046..1588224	+	179	NuclAT_0	-	Antitoxin
-	1588046..1588224	+	179	NuclAT_0	-	Antitoxin
-	1588046..1588224	+	179	NuclAT_0	-	Antitoxin
GIX96_RS07875	1588268..1588417	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
GIX96_RS07880	1588473..1589282	-	810	WP_000166313.1	recombination protein RecT	-
GIX96_RS07885	1589275..1591875	-	2601	WP_000105140.1	exodeoxyribonuclease VIII	-
GIX96_RS07890	1591977..1592252	-	276	WP_001344816.1	hypothetical protein	-
GIX96_RS07895	1592327..1592497	-	171	WP_001352098.1	conserved protein, Rac prophage	-
GIX96_RS07900	1592497..1592718	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1574227..1635545	61318

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T142867 WP_001443846.1 NZ_CP045863:c1588417-1588268 [Escherichia coli O157:H7]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T142867 NZ_CP060892:c797868-797494 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT142867 NZ_CP045863:1588046-1588224 [Escherichia coli O157:H7]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin