Detailed information of TA system TA142862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 61875..62569
• Replicon: chromosome
• Accession: NZ_CP045863
• Organism: Escherichia coli O157:H7 strain ATCC 43890

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: GIX96_RS00300
• Protein ID: WP_001263495.1
• Coordinates: 62171..62569 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: GIX96_RS00295
• Protein ID: WP_000554757.1
• Coordinates: 61875..62168 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GIX96_RS00275	57586..58083	+	498	Protein_47	REP-associated tyrosine transposase RayT	-
GIX96_RS00280	58228..59940	-	1713	Protein_48	flagellar biosynthesis protein FlhA	-
GIX96_RS00285	59912..60697	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
GIX96_RS00290	60768..61823	+	1056	WP_001226188.1	DNA polymerase IV	-
GIX96_RS00295	61875..62168	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
GIX96_RS00300	62171..62569	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
GIX96_RS00305	62579..63031	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
GIX96_RS00310	63350..63553	+	204	Protein_54	RNA ligase RtcB family protein	-
GIX96_RS00315	63552..64073	+	522	Protein_55	peptide chain release factor H	-
GIX96_RS00320	64130..65587	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
GIX96_RS00325	65848..66306	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	66902..66982	+	81	NuclAT_12	-	-
-	66902..66982	+	81	NuclAT_12	-	-
-	66902..66982	+	81	NuclAT_12	-	-
-	66902..66982	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T142862 WP_001263495.1 NZ_CP045863:62171-62569 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T142862 NZ_CP060887:41238-41390 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT142862 WP_000554757.1 NZ_CP045863:61875-62168 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT142862 NZ_CP060887:c41186-41126 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Antitoxin

• AlphaFold DB: S1FJN6