Detailed information of TA system TA142814

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4776557..4777311
• Replicon: chromosome
• Accession: NZ_CP045840
• Organism: Citrobacter sp. S40-1-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5B0T5Y1
• Locus tag: GHC23_RS22675
• Protein ID: WP_048215202.1
• Coordinates: 4776557..4776868 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GHC23_RS22680
• Protein ID: WP_048215203.1
• Coordinates: 4776865..4777311 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHC23_RS22645	4772224..4773126	+	903	WP_003825273.1	formate dehydrogenase O subunit beta	-
GHC23_RS22650	4773123..4773758	+	636	WP_003028686.1	formate dehydrogenase cytochrome b556 subunit	-
GHC23_RS22655	4773755..4774684	+	930	WP_003028685.1	formate dehydrogenase accessory protein FdhE	-
GHC23_RS22660	4774721..4775095	-	375	WP_020996118.1	type II toxin-antitoxin system VapC family toxin	-
GHC23_RS22665	4775095..4775337	-	243	WP_020996119.1	ribbon-helix-helix domain-containing protein	-
GHC23_RS22670	4775543..4776472	+	930	WP_103285937.1	alpha/beta hydrolase	-
GHC23_RS22675	4776557..4776868	+	312	WP_048215202.1	type II toxin-antitoxin system HigB family toxin	Toxin
GHC23_RS22680	4776865..4777311	+	447	WP_048215203.1	helix-turn-helix domain-containing protein	Antitoxin
GHC23_RS22685	4777329..4778270	-	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
GHC23_RS22690	4778315..4778752	-	438	WP_003825289.1	D-tyrosyl-tRNA(Tyr) deacylase	-
GHC23_RS22695	4778749..4779621	-	873	WP_008786998.1	virulence factor BrkB family protein	-
GHC23_RS22700	4779615..4780214	-	600	WP_153753923.1	glucose-1-phosphatase	-
GHC23_RS22705	4780305..4781195	-	891	WP_153753924.1	aldose 1-epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12345.28 Da
• Isoelectric Point: 9.5337

>T142814 WP_048215202.1 NZ_CP045840:4776557-4776868 [Citrobacter sp. S40-1-2]
VHVISRKPFNEAMLMYPNHQLALADLLNVLEKKTFIQPEEMKQYIPSLDNFKYRDKWWVIDVSGNSLRLISYIDFRSHKI
FVKHIVSHAEYDKLTAYYRGNKE

Nucleotide

• Length: 312 bp

>T142814 NZ_CP060873:c4317070-4316897 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16660.97 Da
• Isoelectric Point: 6.7580

>AT142814 WP_048215203.1 NZ_CP045840:4776865-4777311 [Citrobacter sp. S40-1-2]
MRTHRHMDAASAKKIVDTFSDAVKTIPLLGEDRNDTEYRRALALVEYLVDHDDLENPLFELLCARISKYEKHAPEFKALN
QHLEKTPSGVSVLRTLMDQYSLKAADLANELGSKSNVSNILNGRRALTVSHIKALAERFNLPADAFIE

Nucleotide

• Length: 447 bp

>AT142814 NZ_CP060873:c4317394-4317098 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A5B0T5Y1