Detailed information of TA system TA142786

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2498998..2499560
• Replicon: chromosome
• Accession: NZ_CP045837
• Organism: Citrobacter sp. H12-3-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GHC22_RS11945
• Protein ID: WP_153753041.1
• Coordinates: 2499282..2499560 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A6H3AUF8
• Locus tag: GHC22_RS11940
• Protein ID: WP_003833018.1
• Coordinates: 2498998..2499282 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHC22_RS11925	2496693..2497577	+	885	WP_003833024.1	formate dehydrogenase N subunit beta	-
GHC22_RS11930	2497570..2498226	+	657	WP_003020054.1	formate dehydrogenase-N subunit gamma	-
GHC22_RS11935	2498356..2498958	+	603	WP_003833020.1	inorganic diphosphatase	-
GHC22_RS11940	2498998..2499282	-	285	WP_003833018.1	HigA family addiction module antidote protein	Antitoxin
GHC22_RS11945	2499282..2499560	-	279	WP_153753041.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GHC22_RS11950	2499781..2501496	-	1716	WP_153753044.1	ATP-binding cassette domain-containing protein	-
GHC22_RS11955	2501806..2503503	-	1698	WP_003833011.1	malate dehydrogenase	-
GHC22_RS11960	2503683..2503823	-	141	WP_003833009.1	stationary-phase-induced ribosome-associated protein	-
GHC22_RS11965	2504051..2504482	+	432	WP_153753046.1	OsmC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-83)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10666.32 Da
• Isoelectric Point: 10.1807

>T142786 WP_153753041.1 NZ_CP045837:c2499560-2499282 [Citrobacter sp. H12-3-2]
MIMNFRHKGLRDLFLHGRTSGVMAKHVRRLRHRLAVINAASKVTDINMPGYKLHPLVGERDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Nucleotide

• Length: 279 bp

>T142786 NZ_CP060867:c3744997-3744590 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10371.10 Da
• Isoelectric Point: 8.8094

>AT142786 WP_003833018.1 NZ_CP045837:c2499282-2498998 [Citrobacter sp. H12-3-2]
MKMANHPRPGDIIQEALEELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQNTWSLAEAQ
KSVDISRLRKLAVQ

Nucleotide

• Length: 285 bp

>AT142786 NZ_CP060867:c3745244-3744978 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A6H3AUF8