Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 337213..337879 Replicon chromosome
Accession NZ_CP045837
Organism Citrobacter sp. H12-3-2

Toxin (Protein)


Gene name higB Uniprot ID A0A5B0T6K1
Locus tag GHC22_RS01575 Protein ID WP_047408175.1
Coordinates 337562..337879 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A5B0T6F5
Locus tag GHC22_RS01570 Protein ID WP_048236945.1
Coordinates 337213..337509 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GHC22_RS01555 333658..334131 - 474 WP_003023524.1 transcription elongation factor GreB -
GHC22_RS01560 334357..335076 + 720 WP_001157751.1 two-component system response regulator OmpR -
GHC22_RS01565 335073..336425 + 1353 WP_003827658.1 two-component system sensor histidine kinase EnvZ -
GHC22_RS01570 337213..337509 - 297 WP_048236945.1 helix-turn-helix domain-containing protein Antitoxin
GHC22_RS01575 337562..337879 - 318 WP_047408175.1 toxin HigB-2 Toxin
GHC22_RS01580 338002..339624 - 1623 WP_003023529.1 phosphoenolpyruvate carboxykinase (ATP) -
GHC22_RS01585 340003..341721 + 1719 WP_153752095.1 DUF4153 domain-containing protein -
GHC22_RS01590 341831..342709 - 879 WP_153752097.1 Hsp33 family molecular chaperone HslO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12255.17 Da        Isoelectric Point: 10.0909

>T142774 WP_047408175.1 NZ_CP045837:c337879-337562 [Citrobacter sp. H12-3-2]
MFTFIELQGFSKRRPLLLPDDEFRAFQETLIENPEAGDTIAGTGGFRKIRWSRSGMGKRSGIRVIYYNVTRKGRIYLALL
YPKNEQDDLTEEQKRVLMHLSNMLI

Download         Length: 318 bp

>T142774 NZ_CP060867:887168-887566 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11185.95 Da        Isoelectric Point: 7.2052

>AT142774 WP_048236945.1 NZ_CP045837:c337509-337213 [Citrobacter sp. H12-3-2]
MKDELFADLLASAEEMVRIEKGEETPKPEHVHTFSEIDVKAIREATGLRQQDFAIAVGVSYDLVKSWETKRRQPTGAPRK
LLLLIQRNPFIINQLKAI

Download         Length: 297 bp

>AT142774 NZ_CP060867:886872-887165 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5B0T6K1

Antitoxin

Source ID Structure
AlphaFold DB A0A5B0T6F5

References