Detailed information of TA system TA142768

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5362704..5363306
• Replicon: chromosome
• Accession: NZ_CP045827
• Organism: Escherichia coli strain AUSMDU00014361

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: GIJ02_RS26960
• Protein ID: WP_000897305.1
• Coordinates: 5362995..5363306 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GIJ02_RS26955
• Protein ID: WP_000356397.1
• Coordinates: 5362704..5362994 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GIJ02_RS26930	5358629..5359531	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
GIJ02_RS26935	5359528..5360163	+	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-
GIJ02_RS26940	5360160..5361089	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
GIJ02_RS26945	5361420..5361662	-	243	WP_001086388.1	hypothetical protein	-
GIJ02_RS26950	5361881..5362099	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
GIJ02_RS26955	5362704..5362994	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
GIJ02_RS26960	5362995..5363306	-	312	WP_000897305.1	hypothetical protein	Toxin
GIJ02_RS26965	5363535..5364443	+	909	WP_001162704.1	alpha/beta hydrolase	-
GIJ02_RS26970	5364507..5365448	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
GIJ02_RS26975	5365493..5365930	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
GIJ02_RS26980	5365927..5366799	-	873	WP_000920762.1	virulence factor BrkB family protein	-
GIJ02_RS26985	5366793..5367392	-	600	WP_001315111.1	glucose-1-phosphatase	-
GIJ02_RS26990	5367491..5368276	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T142768 WP_000897305.1 NZ_CP045827:c5363306-5362995 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T142768 NZ_CP060863:c248578-248306 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT142768 WP_000356397.1 NZ_CP045827:c5362994-5362704 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT142768 NZ_CP060863:c248841-248578 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7