Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4392067..4392602 Replicon chromosome
Accession NZ_CP045827
Organism Escherichia coli strain AUSMDU00014361

Toxin (Protein)


Gene name yafQ Uniprot ID A0A0F3UZU8
Locus tag GIJ02_RS22365 Protein ID WP_032183258.1
Coordinates 4392315..4392602 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag GIJ02_RS22360 Protein ID WP_000729704.1
Coordinates 4392067..4392327 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GIJ02_RS22335 4387104..4388243 + 1140 WP_000785656.1 flagellar type III secretion system protein FlhB -
GIJ02_RS22340 4388227..4390319 + 2093 Protein_4384 flagellar biosynthesis protein FlhA -
GIJ02_RS22345 4390424..4390702 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
GIJ02_RS22350 4390695..4391051 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
GIJ02_RS22355 4391108..4391881 - 774 WP_000543897.1 C40 family peptidase -
GIJ02_RS22360 4392067..4392327 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
GIJ02_RS22365 4392315..4392602 + 288 WP_032183258.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
GIJ02_RS22370 4392758..4393498 + 741 WP_001225679.1 murein L,D-transpeptidase -
GIJ02_RS22375 4393469..4394236 - 768 WP_000333380.1 class II glutamine amidotransferase -
GIJ02_RS22380 4394442..4395020 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(7-94)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11166.96 Da        Isoelectric Point: 10.2267

>T142765 WP_032183258.1 NZ_CP045827:4392315-4392602 [Escherichia coli]
IRNLNQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDK
LLRFERTGTHAALFG

Download         Length: 288 bp

>T142765 NZ_CP060861:498673-499014 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT142765 WP_000729704.1 NZ_CP045827:4392067-4392327 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT142765 NZ_CP060861:498443-498673 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F3UZU8

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References