Detailed information of TA system TA142741

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1094035..1094762
• Replicon: chromosome
• Accession: NZ_CP045827
• Organism: Escherichia coli strain AUSMDU00014361

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UEW4
• Locus tag: GIJ02_RS05275
• Protein ID: WP_000547559.1
• Coordinates: 1094035..1094346 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GIJ02_RS05280
• Protein ID: WP_000126294.1
• Coordinates: 1094343..1094762 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GIJ02_RS05245	1089177..1090886	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
GIJ02_RS05250	1090896..1091438	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
GIJ02_RS05255	1091438..1092205	+	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
GIJ02_RS05260	1092202..1092612	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
GIJ02_RS05265	1092605..1093075	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
GIJ02_RS05270	1093118..1093861	+	744	WP_001341829.1	hypothetical protein	-
GIJ02_RS05275	1094035..1094346	+	312	WP_000547559.1	type II toxin-antitoxin system HigB family toxin	Toxin
GIJ02_RS05280	1094343..1094762	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
GIJ02_RS05285	1094876..1096300	-	1425	WP_000110313.1	6-phospho-beta-glucosidase AscB	-
GIJ02_RS05290	1096309..1097766	-	1458	WP_001107856.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
GIJ02_RS05295	1098026..1099036	+	1011	WP_001377591.1	DNA-binding transcriptional regulator AscG	-
GIJ02_RS05300	1099185..1099712	+	528	WP_001078781.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (24-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12450.11 Da
• Isoelectric Point: 9.7249

>T142741 WP_000547559.1 NZ_CP045827:1094035-1094346 [Escherichia coli]
MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T142741 NZ_CP060849:c2543013-2542795 [Salmonella enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT142741 WP_000126294.1 NZ_CP045827:1094343-1094762 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT142741 NZ_CP060849:c2543415-2543041 [Salmonella enterica]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A0F3V163