Detailed information of TA system TA142616

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 51116..51804
• Replicon: plasmid p1
• Accession: NZ_CP045807
• Organism: Gordonia pseudamarae strain BEN371

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GII33_RS22955
• Protein ID: WP_213250998.1
• Coordinates: 51116..51469 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GII33_RS22960
• Protein ID: WP_213251000.1
• Coordinates: 51490..51804 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GII33_RS22930 (GII33_22920)	46401..47249	+	849	WP_066903545.1	sialidase family protein	-
GII33_RS22935 (GII33_22925)	47653..47859	+	207	WP_213250993.1	heavy metal-associated domain-containing protein	-
GII33_RS22940 (GII33_22930)	47912..48082	+	171	WP_213251228.1	hypothetical protein	-
GII33_RS22945 (GII33_22935)	48163..50370	+	2208	WP_213251212.1	heavy metal translocating P-type ATPase	-
GII33_RS22950 (GII33_22940)	50662..51009	+	348	WP_213250996.1	metal-sensitive transcriptional regulator	-
GII33_RS22955 (GII33_22945)	51116..51469	+	354	WP_213250998.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GII33_RS22960 (GII33_22950)	51490..51804	+	315	WP_213251000.1	helix-turn-helix transcriptional regulator	Antitoxin
GII33_RS22965 (GII33_22955)	51977..52507	-	531	WP_246222382.1	DUF2269 domain-containing protein	-
GII33_RS22970 (GII33_22960)	52510..52743	-	234	WP_213251006.1	hypothetical protein	-
GII33_RS22975 (GII33_22965)	52940..54730	-	1791	WP_213251009.1	arsenical pump-driving ATPase	-
GII33_RS22980 (GII33_22970)	54788..55186	-	399	WP_213251011.1	arsenite efflux transporter metallochaperone ArsD	-
GII33_RS22985 (GII33_22975)	55224..55646	-	423	WP_213251013.1	low molecular weight phosphatase family protein	-
GII33_RS22990 (GII33_22980)	55700..56107	+	408	WP_213251016.1	metalloregulator ArsR/SmtB family transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..93188	93188

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (20-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-91)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13549.37 Da
• Isoelectric Point: 4.3715

>T142616 WP_213250998.1 NZ_CP045807:51116-51469 [Gordonia pseudamarae]
MECEVVLLDDVEQWYLRLVESDDDDAAAVTAAIDYLEREGPTLGRPVVDKVKGSRVHNMKELRPSGTSIRILFVFDPQRQ
AVLLIAGDKAGEWKQWYIDNIPIAEDRYNMWLEEQKR

Nucleotide

• Length: 354 bp

>T142616 NZ_CP060789:c1089396-1089130 [Tessaracoccus defluvii]
GTGAGTCCCTGGACTCTGGTGTACTCACGCCAAGCCCAGAAGGACGCCCAGAAGCTGGCCTCCTCAGGGCTGAAGACGAA
GGCGCAGCACCTGCTGGAGGTTCTGGCCCAGGACCCGTTCACCACGCCACCCAAGTACGAGAAGTTGGTCGGCGACCTCG
CTGGCTGCTACTCACGGCGCATCAACATCCAGCACCGACTTGTCTATGAGGTGCTCCCTGATCGCCACGTTGTCCACATC
CTGAGGATGTGGACCCACTACGAGTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11771.12 Da
• Isoelectric Point: 5.6172

>AT142616 WP_213251000.1 NZ_CP045807:51490-51804 [Gordonia pseudamarae]
MARHWREVRAEVAEKLDEQDVSNASKTMQEEVRAHRLVEIRREQGWTRQDAVAEVMGISQSRVSQIERGDLRHTQLGTLE
SYVEALGGHLRVVADFGDSSLTLG

Nucleotide

• Length: 315 bp

>AT142616 NZ_CP060789:c1089635-1089393 [Tessaracoccus defluvii]
ATGACTGCTGTGAGCGCAACGTCTGCCCGCGCGAACCTGTACCGGTTGATCGACCAGGTCAACGAGGAGTCCGAGCCGCT
GACCATCACCGGCCAGCGTGGCAACGCCGTGCTCATCGGCGAGGAGGACTGGCTCGCCATCCAGGAGACGCTGTTCCTTG
AGTCGGTACCGGGGTTGGCCGATTCCATCCGGCGCTCCCGCGCGGAGGGTGTCGACGCGGGCTCGACCGAGCTTGACTGG
TGA