Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2965221..2965948 Replicon chromosome
Accession NZ_CP045784
Organism Shigella dysenteriae strain CFSAN029786

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag GH770_RS15035 Protein ID WP_000550189.1
Coordinates 2965221..2965535 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag GH770_RS15040 Protein ID WP_000560263.1
Coordinates 2965532..2965948 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GH770_RS15015 (2961387) 2961387..2962373 - 987 WP_004986115.1 Gfo/Idh/MocA family oxidoreductase -
GH770_RS15020 (2962452) 2962452..2963135 - 684 WP_001183035.1 vancomycin high temperature exclusion protein -
GH770_RS15025 (2963212) 2963212..2963715 - 504 WP_005043640.1 M48 family metallopeptidase -
GH770_RS15030 (2963800) 2963800..2964936 + 1137 WP_000018665.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
GH770_RS15035 (2965221) 2965221..2965535 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
GH770_RS15040 (2965532) 2965532..2965948 + 417 WP_000560263.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
GH770_RS15045 (2965993) 2965993..2968011 - 2019 WP_000121440.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
GH770_RS15050 (2968237) 2968237..2970588 - 2352 Protein_2954 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T142575 WP_000550189.1 NZ_CP045784:2965221-2965535 [Shigella dysenteriae]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T142575 NZ_CP060737:c4141974-4141633 [Enterobacter roggenkampii]
ATGAATACTTTACCTGCAACAACCCAGCGGGCGGCGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGCTGCTGAC
CCGACTGCTCGAAAAGCACTATGGTCTGACCCTCAACGACACCCCGTTCAGCGATGAAACGGTTATTCGGGAACATATTA
ATGCTGGTATTACACTGGCTGATGCAGTGAATTTTCTGGTGGAAAAATACGAACTGGTCCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCACCTTATCTTCGGGCTGTAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAATTCGGTACAGTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14979.42 Da        Isoelectric Point: 4.4547

>AT142575 WP_000560263.1 NZ_CP045784:2965532-2965948 [Shigella dysenteriae]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLADLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT142575 NZ_CP060737:c4142312-4141995 [Enterobacter roggenkampii]
ATGAGCAATATCACATGGGGCCTGCAGCGGGCTATCACGCCGTGCCTGGGAGCTCGTCTGGTGCAGGAGGGTAACCGACT
GCACTACCTAGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACGCCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAAGTCAGCCCCCAACATGCCCACTGCGTCATCCTGTACCAC
AACGGTTTCACCTGTGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References