Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2607426..2608072 Replicon chromosome
Accession NZ_CP045783
Organism Klebsiella variicola strain LEMB11

Toxin (Protein)


Gene name higB Uniprot ID A0A1F2M0N0
Locus tag GFC07_RS12620 Protein ID WP_008806991.1
Coordinates 2607725..2608072 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1F2LZT5
Locus tag GFC07_RS12615 Protein ID WP_008806992.1
Coordinates 2607426..2607725 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GFC07_RS12595 2603632..2604390 - 759 WP_008806995.1 DeoR/GlpR family transcriptional regulator -
GFC07_RS12600 2604440..2605270 - 831 WP_008806994.1 rhomboid family intramembrane serine protease GlpG -
GFC07_RS12605 2605321..2605650 - 330 WP_004202138.1 thiosulfate sulfurtransferase GlpE -
GFC07_RS12610 2605855..2607363 + 1509 WP_004202136.1 glycerol-3-phosphate dehydrogenase -
GFC07_RS12615 2607426..2607725 - 300 WP_008806992.1 helix-turn-helix transcriptional regulator Antitoxin
GFC07_RS12620 2607725..2608072 - 348 WP_008806991.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GFC07_RS12625 2608287..2610734 - 2448 WP_008806990.1 glycogen phosphorylase -
GFC07_RS12630 2610752..2612185 - 1434 WP_004202133.1 glycogen synthase GlgA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13504.52 Da        Isoelectric Point: 6.2327

>T142562 WP_008806991.1 NZ_CP045783:c2608072-2607725 [Klebsiella variicola]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFGPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T142562 NZ_CP060733:c3917211-3916801 [Enterobacter roggenkampii]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCGCAGTGGATGTCTTTATTGCTCCACGGCCTGGTTGCGGC
CTTTGTGTTACTGATGCCGTGGCCGCTGAGCTATACGCCGCTGTGGCTGCTGCTACTTTCGTTTGTCGTTTTTGACAGCG
TACGCAGCCAGCGTCGCATTAATGCCCGTCAGGGAGAGATCAAGTTACTGATGGATTCCCGCCTGCGCTGGCAGGGAAAA
GAGTGGGATATTCTCGGTATGCCCTGGATGCTCAACTGCGGCATGATGTTACGGTTACGCAAGGTGGACGGCGGGCGGTG
CCAGCATCTTTGGCTGGCGGCTGACAGTATGGATGCTGCCGAATGGCGAGACCTGCGCCGGATGCTGTTGCAGCAGACGA
CGCAGGGGTAG

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10814.61 Da        Isoelectric Point: 10.1464

>AT142562 WP_008806992.1 NZ_CP045783:c2607725-2607426 [Klebsiella variicola]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT142562 NZ_CP060733:c3917458-3917192 [Enterobacter roggenkampii]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATTTCCATCATGCCTTT
CTTCGAATATGAGTATGACACCTTAAGCGACGACGATAAGCGTCTGTTTGTTCGCCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAACCACGGCAAACCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M0N0

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZT5

References