Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4608038..4608635 Replicon chromosome
Accession NZ_CP045782
Organism Klebsiella pneumoniae strain LEMB18

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag GFB99_RS22105 Protein ID WP_004142563.1
Coordinates 4608038..4608355 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag GFB99_RS22110 Protein ID WP_004142561.1
Coordinates 4608348..4608635 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GFB99_RS22090 4603741..4605168 - 1428 WP_102001330.1 MFS transporter -
GFB99_RS22095 4605277..4606143 + 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
GFB99_RS22100 4606810..4607853 + 1044 WP_023284821.1 DUF2157 domain-containing protein -
GFB99_RS22105 4608038..4608355 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GFB99_RS22110 4608348..4608635 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
GFB99_RS22115 4608900..4609553 - 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
GFB99_RS22120 4609673..4610041 - 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
GFB99_RS22125 4610041..4610622 - 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
GFB99_RS22130 4610802..4611917 + 1116 WP_020316605.1 MBL fold metallo-hydrolase -
GFB99_RS22135 4611948..4612289 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
GFB99_RS22140 4612307..4612555 - 249 WP_002893037.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T142550 WP_004142563.1 NZ_CP045782:4608038-4608355 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T142550 NZ_CP060726:c4501743-4501441 [Providencia rettgeri]
ATGATCACGGTTCTAACAACGGAATGTTTTGATAGTTGGATCAAAAATCTTAGAGATATTCGAGCTAAAACTAAAATTCT
AATGAGAATTAGGCGGTTAAAAAATGGAAATTATGGTGATGTTCAACCCATTGGTGATGGCTTTTCGGAACTGAGGGTAC
ATGAAGGACAAGGGTATCGTGTTTATCTAAAGCAGAAAAACAATGTTATTGTAATTTTACTTTGTGGTGGGACAAAGGCA
ACGCAGCAGAAGGATATCCATAAAGCTAAACTCCTTTTTGGTGAAGTTGAGGGGGAACTATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT142550 WP_004142561.1 NZ_CP045782:4608348-4608635 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT142550 NZ_CP060726:c4501444-4501160 [Providencia rettgeri]
ATGAATACTAAATTAAAAGAATTTGATATCGCTGAGTATTTAAACAGTGAAGAAGAAATACAAATGTATCTAAACGAAAT
TCTAGAAGAAGGTGACCCTAGTCTAGTTCTCGCTGCACTTGGTGATATTGCGAAGGCAAGAAATATGAGCCAATTAGCTA
AAGAAGTTGGAATGAGTCGTGAAGGTTTATATAAGGCGCTATCAGGACAGGGAAATCCAACATTTATGACGATTATAAAA
ATCGTTAATTCTCTCGGATTAAAGCTACAGTTTAAGAGTATCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References