Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 2873694..2874280 Replicon chromosome
Accession NZ_CP045782
Organism Klebsiella pneumoniae strain LEMB18

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag GFB99_RS14055 Protein ID WP_002920800.1
Coordinates 2873694..2874062 (-) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag GFB99_RS14060 Protein ID WP_004174006.1
Coordinates 2874059..2874280 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GFB99_RS14035 2869197..2870267 - 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -
GFB99_RS14040 2870269..2871114 - 846 WP_004185988.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
GFB99_RS14045 2871111..2871998 - 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
GFB99_RS14050 2872105..2873421 - 1317 WP_032435158.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
GFB99_RS14055 2873694..2874062 - 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
GFB99_RS14060 2874059..2874280 - 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
GFB99_RS14065 2874444..2875157 - 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
GFB99_RS14070 2875159..2875926 - 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
GFB99_RS14075 2875923..2877200 - 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
GFB99_RS14080 2877197..2878123 - 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2868460..2889047 20587


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T142544 WP_002920800.1 NZ_CP045782:c2874062-2873694 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T142544 NZ_CP060726:c1783425-1783240 [Providencia rettgeri]
GTGCAAAGTTCGGAACTGATAAACATACTGCAAAAAAACGGTTGGAAACTTGAAAGAATAAAAGGAAGTCACCATCAGTT
TTCCCACCCACATTTTTCAATTGTAATTACTGTTCCGCATCCACAAAAAGATTTAAAGATAGGCACACTAAATCACATTT
TGAAGGCTGCGAAACTAAAACATTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT142544 WP_004174006.1 NZ_CP045782:c2874280-2874059 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT142544 NZ_CP060726:c1783190-1782777 [Providencia rettgeri]
ATGCTTTATACCGCCTTTATTGAAATTGATAATGATGGCAGCGCAAGTGGCTGGTTTCCTGATATTGAAGGATGTACATT
CGCGGGCAGCAATATAGAAGAGGCTTACGCAGAAGCAAAATCAGCAATTGATGCTCATTTTGAATTATTGAGTGAAAAAG
GCTTTGAGATCCCATTATCGAAATCTCAGCAAACGCTATTGAACCCAATACAACCTGAATATGCGCATGGGATATGGTTA
TTTGTGGATGTCGATATCGATAAATACGATGGCCGTACTGAGCGTATTAATATCACCTTGCCGCATCGTTTATTACATCG
TATTGATGCTTTAGTGAAGGTTAATCCTGAATATGGTAGCCGTAGTGGTTTTATTGCTGTTGCAGCTCGTAAAGAATTAA
AAAAAACCGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References