Detailed information of TA system TA142543

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2847416..2848092
• Replicon: chromosome
• Accession: NZ_CP045782
• Organism: Klebsiella pneumoniae strain LEMB18

Toxin (Protein)

• Gene name: higB
• Uniprot ID: W9BBY1
• Locus tag: GFB99_RS13945
• Protein ID: WP_016529833.1
• Coordinates: 2847745..2848092 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GFB99_RS13940
• Protein ID: WP_032413101.1
• Coordinates: 2847416..2847745 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GFB99_RS13920	2843652..2844410	-	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-
GFB99_RS13925	2844460..2845290	-	831	WP_004151408.1	rhomboid family intramembrane serine protease GlpG	-
GFB99_RS13930	2845341..2845670	-	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
GFB99_RS13935	2845875..2847383	+	1509	WP_002920554.1	glycerol-3-phosphate dehydrogenase	-
GFB99_RS13940	2847416..2847745	-	330	WP_032413101.1	helix-turn-helix transcriptional regulator	Antitoxin
GFB99_RS13945	2847745..2848092	-	348	WP_016529833.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GFB99_RS13950	2848268..2850715	-	2448	WP_102001163.1	glycogen phosphorylase	-
GFB99_RS13955	2850733..2852166	-	1434	WP_002920564.1	glycogen synthase GlgA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (9-87)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13549.56 Da
• Isoelectric Point: 5.6749

>T142543 WP_016529833.1 NZ_CP045782:c2848092-2847745 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T142543 NZ_CP060726:1507639-1508049 [Providencia rettgeri]
GTGGTCCTGTGGAAATCTAATCTCTCCATTTCATGGAAAACCCAACTGTTCTCAACCTGTGTTCATGGGGTCATTGGGAT
GTTTTTACTATTGGCTCCATGGTCCCCTGGTAACTCAATGATATGGTTGCCGCTATTAGTGGTTGTCGTTGCGAGTTGGG
CAAAGAGCCAGAAAAATATCAGTAAAATAAAAGGGGTTGCTGTACTGGTTAATGGCAATAAGGTTCAGTGGAAGAAAAAT
GAATGGAGAATACTGAAAGCACCTTGGCTTACCCGCTATGGTATATTACTGACACTAGAAGCGTTACAAGGTAAACCACA
AAAGCTCCATTTGTGGGTTGCAAAAGACGCATTGTCGGAAGAGAATTGGCGTAATTTAAACCAATTATTGCTGCAATACC
CTGATATTTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11921.98 Da
• Isoelectric Point: 10.8046

>AT142543 WP_032413101.1 NZ_CP045782:c2847745-2847416 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNLLTGIRAKRKP

Nucleotide

• Length: 330 bp

>AT142543 NZ_CP060726:1507392-1507658 [Providencia rettgeri]
ATGGATATTGAGAATAAAGCGCGAATTCATTGGGCATGCCGCCGTGGTATGCGTGAATTAGATATTTCAATCATGCCGTT
TTTTGAGTATGAATATGATGACCTAACAACTCACGATAAGCAGGTATTTGTCCGTTTATTAGAATGTGCGGACCCTGATT
TATTCAATTGGCTAATGAATCACGGTCGTCCAGATGATGACGAACTTTTTCGTATGATTAAACTGATTCAGGAAAAAAAT
AAAAATCGTGGTCCTGTGGAAATCTAA

Structures

Toxin

• AlphaFold DB: W9BBY1