Detailed information of TA system TA142532

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 830517..831206
• Replicon: chromosome
• Accession: NZ_CP045782
• Organism: Klebsiella pneumoniae strain LEMB18

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A331C6E2
• Locus tag: GFB99_RS04135
• Protein ID: WP_021469727.1
• Coordinates: 830889..831206 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GFB99_RS04130
• Protein ID: WP_040147778.1
• Coordinates: 830517..830813 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GFB99_RS04115	826909..828291	+	1383	WP_004151222.1	MFS transporter	-
GFB99_RS04120	828335..829951	+	1617	WP_040147777.1	carbohydrate porin	-
GFB99_RS04125	829992..830438	-	447	WP_004175959.1	hypothetical protein	-
GFB99_RS04130	830517..830813	-	297	WP_040147778.1	helix-turn-helix domain-containing protein	Antitoxin
GFB99_RS04135	830889..831206	-	318	WP_021469727.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GFB99_RS04140	831413..831640	-	228	WP_002906690.1	tautomerase PptA	-
GFB99_RS04145	831711..832328	-	618	WP_004175955.1	glutathione S-transferase family protein	-
GFB99_RS04150	832406..833257	-	852	WP_014343117.1	transporter substrate-binding domain-containing protein	-
GFB99_RS04155	833296..834075	-	780	WP_002906697.1	amino acid ABC transporter ATP-binding protein	-
GFB99_RS04160	834059..834985	-	927	WP_004189808.1	amino acid ABC transporter permease	-
GFB99_RS04165	834995..835504	-	510	WP_002906702.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-76)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12221.10 Da
• Isoelectric Point: 10.2217

>T142532 WP_021469727.1 NZ_CP045782:c831206-830889 [Klebsiella pneumoniae]
MLTFIELQGFSKRRQVLLQDDEFRAFQEILINDPEAGDTISGTGGFRKIRWSRPGMGKRGGVRVIYYNVTKKGRIYLALI
YPKNEQDELNQEQKKMLRQVADLIN

Nucleotide

• Length: 318 bp

>T142532 NZ_CP060716:c949459-949178 [Leucobacter denitrificans]
ATGATTAGATCGTTCGGTAATAAGGACACTGAGCGATTGTGGCGTAGGGAACGGGTAAGGTCGATCGATCCTCGGATTCA
TTCGGTTGCGCTACGGAAGCTGCGCCAGGTGGAAGCAGCGGTGTCACTCGAAGACCTCCGGATTCCGCCAGGGAACCGGC
TCGAATCACTGAAAGGCGACAGGGTCGGAGAACATAGCATCAGGGTTATTGATCAATGGCGGATTTGCTTCGTGTGGACC
GCAACTGGGCCAGAAAGGGTTGAAATCGTTGACTACAATTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11032.88 Da
• Isoelectric Point: 6.4721

>AT142532 WP_040147778.1 NZ_CP045782:c830813-830517 [Klebsiella pneumoniae]
MKDELFADLLASAEEMVRIEKGEQMPMEQHVHTFSEIDVKAIREATGLKQQDFAAAVGVSYELVKSWETKRRQPTGAARK
LLLLLQGNPLIINLLKVV

Nucleotide

• Length: 297 bp

>AT142532 NZ_CP060716:c949191-948865 [Leucobacter denitrificans]
TTGACTACAATTGACAAGCTCTCCCCGATTCATCCGGGTGAGGTGCTCATGGAGGACTTCATCACCGGTTTTGGTATCAC
ACAACACAAGCTAGCCGTGTCAATCGGGGTGCCCCCGCGTCGCATTAATGAAATTGTGCACGGTAAGCGCTCGATTACAG
CAGATACTGCGCTTCGTCTGAGTAGATATTTTGGTGTGTCTGCACAGTTCTGGATGAATCTGCAGGTGCGCTACGATCTC
GACCTTGCTGAGGATCGCGTTGCCGATGAGATCGCCGAAATCGCTCCTTTGCGTGCCGCCGATGCCTCGCTTGACGTTCA
GTCTTAA

Structures

Toxin

• AlphaFold DB: A0A331C6E2