Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/COG4683-HTH_37 |
| Location | 26589..27246 | Replicon | plasmid pTHNK130-1 |
| Accession | NZ_CP045742 | ||
| Organism | Escherichia coli strain DH5alpha | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | U3PDC3 |
| Locus tag | GHR40_RS22280 | Protein ID | WP_000270043.1 |
| Coordinates | 26896..27246 (-) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | GHR40_RS22275 | Protein ID | WP_000124640.1 |
| Coordinates | 26589..26891 (-) | Length | 101 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| GHR40_RS22225 | 22214..22642 | + | 429 | WP_000591074.1 | hypothetical protein | - |
| GHR40_RS22230 | 22699..23058 | + | 360 | WP_000422768.1 | hypothetical protein | - |
| GHR40_RS22235 | 23058..23504 | + | 447 | WP_000919345.1 | Fe3+-siderophore ABC transporter permease | - |
| GHR40_RS22240 | 23501..24019 | + | 519 | WP_000210756.1 | nitrite reductase | - |
| GHR40_RS22245 | 24019..24249 | + | 231 | WP_000972663.1 | hypothetical protein | - |
| GHR40_RS22250 | 24236..25093 | + | 858 | WP_001167032.1 | hypothetical protein | - |
| GHR40_RS22255 | 25119..25307 | + | 189 | WP_001270411.1 | hypothetical protein | - |
| GHR40_RS22260 | 25324..25851 | + | 528 | WP_001236377.1 | thermonuclease family protein | - |
| GHR40_RS22265 | 25909..26181 | + | 273 | WP_001043047.1 | HU family DNA-binding protein | - |
| GHR40_RS22270 | 26269..26562 | + | 294 | WP_001239997.1 | chromosome segregation protein ParM | - |
| GHR40_RS22275 | 26589..26891 | - | 303 | WP_000124640.1 | XRE family transcriptional regulator | Antitoxin |
| GHR40_RS22280 | 26896..27246 | - | 351 | WP_000270043.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| GHR40_RS22285 | 27409..27957 | + | 549 | WP_001061574.1 | transcriptional regulator | - |
| GHR40_RS22290 | 28298..28492 | + | 195 | WP_000343597.1 | hypothetical protein | - |
| GHR40_RS22295 | 28503..28874 | + | 372 | WP_000516916.1 | hypothetical protein | - |
| GHR40_RS22300 | 28867..29337 | + | 471 | WP_001281821.1 | hypothetical protein | - |
| GHR40_RS22305 | 29352..29687 | - | 336 | WP_000683476.1 | hypothetical protein | - |
| GHR40_RS22310 | 29784..30272 | + | 489 | WP_001273096.1 | DUF1643 domain-containing protein | - |
| GHR40_RS22315 | 30275..30772 | + | 498 | WP_000062185.1 | hypothetical protein | - |
| GHR40_RS22320 | 30987..31691 | - | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | blaCTX-M-14 / fosA3 / floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / aph(3')-IIa / aadA5 | - | 1..180783 | 180783 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 13328.21 Da Isoelectric Point: 5.2421
>T142464 WP_000270043.1 NZ_CP045742:c27246-26896 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
Download Length: 351 bp
>T142464 NZ_CP060704:c2884432-2884058 [Chitinimonas koreensis]
GTGATCCTGGTCGATACGTCGGTCTGGATCGATCACCTGCGGCAGGGCGACGAGCAGTTGCGCAGCCTGCTCGAAAGCGG
TTCGGTCCATGTGCACCCGATGGTGGTCGGCGAGCTGGCCTGCGGCCAGCTGGGTGCGCGGCGGCAGGTGTTGGACCTGC
TGGCCGCCTTGCCGGCGCTGCCGCGCGCGGAGGATGCCGAGGTGCTGTTCTTCATCGAGCAGCGGCGCCTGATGGGGCGC
GGGATCGGTTACGTCGACGCGCACCTGCTGGCGTCGACCGTCATGCAGCCGCCCGCGCGGCTATGGAGCCGCGACAAGCG
CCTGGCCGGCATCGCGCAGGAACTCGGCTGCGCTTTCGCCGAGGCGCAGCATTGA
GTGATCCTGGTCGATACGTCGGTCTGGATCGATCACCTGCGGCAGGGCGACGAGCAGTTGCGCAGCCTGCTCGAAAGCGG
TTCGGTCCATGTGCACCCGATGGTGGTCGGCGAGCTGGCCTGCGGCCAGCTGGGTGCGCGGCGGCAGGTGTTGGACCTGC
TGGCCGCCTTGCCGGCGCTGCCGCGCGCGGAGGATGCCGAGGTGCTGTTCTTCATCGAGCAGCGGCGCCTGATGGGGCGC
GGGATCGGTTACGTCGACGCGCACCTGCTGGCGTCGACCGTCATGCAGCCGCCCGCGCGGCTATGGAGCCGCGACAAGCG
CCTGGCCGGCATCGCGCAGGAACTCGGCTGCGCTTTCGCCGAGGCGCAGCATTGA
Antitoxin
Download Length: 101 a.a. Molecular weight: 11006.73 Da Isoelectric Point: 7.2036
>AT142464 WP_000124640.1 NZ_CP045742:c26891-26589 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
Download Length: 303 bp
>AT142464 NZ_CP060704:c2884626-2884429 [Chitinimonas koreensis]
ATGCGGACCACCCTCAACCTGGACGACGCGCTGCTCGACCAGGCCCAGCGCCTGAGCGGCCTGCAGGAGCGCAGTGCGCT
GATGCGCGAAGCGCTGCTGGCCCTGATCCAGCGCGAAAGCGCGCGCCGCCTGGCCCGCCTCGGCGGTTCCGAGCCCGACC
TGCAGGCCGTGCCGCGGCGCAGGGACGGCGAGCCGTGA
ATGCGGACCACCCTCAACCTGGACGACGCGCTGCTCGACCAGGCCCAGCGCCTGAGCGGCCTGCAGGAGCGCAGTGCGCT
GATGCGCGAAGCGCTGCTGGCCCTGATCCAGCGCGAAAGCGCGCGCCGCCTGGCCCGCCTCGGCGGTTCCGAGCCCGACC
TGCAGGCCGTGCCGCGGCGCAGGGACGGCGAGCCGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|